6SB2
cryo-EM structure of mTORC1 bound to active RagA/C GTPases
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | mTOR,Serine/threonine-protein kinase mTOR,mTOR,Serine/threonine-protein kinase mTOR | polymer | 2549 | 287235.2 | 2 | UniProt (P42345) Pfam (PF11865) Pfam (PF02259) Pfam (PF08771) Pfam (PF00454) Pfam (PF02260) In PDB | Homo sapiens | FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin,mTOR,Mechanistic target of rapamycin,Rapamycin and FKBP12 target 1,Rapamycin target protein 1,FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin,mTOR,Mechanistic target of rapamycin,Rapamycin and FKBP12 target 1,Rapamycin target protein 1 |
2 | E, H | Target of rapamycin complex subunit LST8 | polymer | 326 | 35910.1 | 2 | UniProt (Q9BVC4) Pfam (PF00400) In PDB | Homo sapiens (Human) | TORC subunit LST8,G protein beta subunit-like,Protein GbetaL,Mammalian lethal with SEC13 protein 8,mLST8 |
3 | C, I | Ras-related GTP-binding protein A | polymer | 313 | 36600.2 | 2 | UniProt (Q7L523) Pfam (PF04670) In PDB | Homo sapiens (Human) | RagA,Adenovirus E3 14.7 kDa-interacting protein 1,FIP-1 |
4 | D, J | Ras-related GTP-binding protein C | polymer | 399 | 44284.8 | 2 | UniProt (Q9HB90) Pfam (PF04670) In PDB | Homo sapiens (Human) | RagC,GTPase-interacting protein 2,TIB929 |
5 | Y, N | Regulatory-associated protein of mTOR | polymer | 1335 | 149200.0 | 2 | UniProt (Q8N122) Pfam (PF14538) Pfam (PF02985) Pfam (PF00400) In PDB | Homo sapiens (Human) | Raptor,p150 target of rapamycin (TOR)-scaffold protein |
6 | C, I | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) | |||
7 | D, J | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) |
Sequence modifications
A, B: -5 - 59 (PDB: 6SB2)
A, B: 60 - 355 (UniProt: P42345)
A, B: 356 - 380 (PDB: 6SB2)
A, B: 381 - 2549 (UniProt: P42345)
C, I: 1 - 313 (UniProt: Q7L523)
D, J: 1 - 399 (UniProt: Q9HB90)
A, B: 60 - 355 (UniProt: P42345)
A, B: 356 - 380 (PDB: 6SB2)
A, B: 381 - 2549 (UniProt: P42345)
C, I: 1 - 313 (UniProt: Q7L523)
PDB | External Database | Details |
---|---|---|
Leu 66 | Gln 66 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Asn 90 | Thr 90 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 10 |
Total formula weight | 1106460.6 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 1932.8 | |
All* | Total formula weight | 1108393.4 |