6GFI

Structure of Human Mesotrypsin in complex with APPI variant T11V/M17R/I18F/F34V

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	PRSS3 protein	polymer	224	24257.5	2	UniProt (Q8N2U3) Pfam (PF00089) UniProt (by SIFTS) (P35030) In PDB	Homo sapiens (Human)
2	E, C	Amyloid-beta A4 protein	polymer	81	9185.0	2	UniProt (P05067) Pfam (PF00014) In PDB	Homo sapiens (Human)	ABPP,APPI,APP,Alzheimer disease amyloid protein,Amyloid precursor protein,Amyloid-beta precursor protein,Cerebral vascular amyloid peptide,CVAP,PreA4,Protease nexin-II,PN-II
3	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
4		water	water		18.0	12	Chemie (HOH)

Sequence modifications

A, B: 16 - 246 (UniProt: Q8N2U3)

PDB	External Database	Details
Ala 195	Ser 204	conflict

E, C: 8 - 60 (UniProt: P05067)

PDB	External Database	Details
Tyr -9	-	expression tag
Val -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Glu 1	-	expression tag
Val 2	-	expression tag
Glu 3	-	expression tag
Val 4	-	expression tag
Cys 5	-	expression tag
Ser 6	-	expression tag
Glu 7	-	expression tag
Val 11	Thr 297	engineered mutation
Arg 17	Met 303	conflict
Phe 18	Ile 304	conflict
Val 34	Phe 320	conflict
Arg 61	-	expression tag
His 62	-	expression tag
His 63	-	expression tag
His 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag
His 67	-	expression tag
Ala 68	-	expression tag
Ala 69	-	expression tag
Ala 70	-	expression tag
Asn 71	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	66885.0
Non-Polymers*	Number of molecules	1
	Total formula weight	62.1
All*	Total formula weight	66947.0

*Water molecules are not included.