6G0A
The crystal structure of the Pol2 catalytic domain of DNA polymerase epsilon carrying a P301R substitution.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | DNA polymerase epsilon catalytic subunit A | polymer | 1191 | 137235.7 | 1 | UniProt (P21951) Pfam (PF03104) Pfam (PF00136) In PDB | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | DNA polymerase II subunit A |
2 | P | DNA (5'-D(P*TP*AP*AP*CP*CP*GP*CP*GP*TP*TP*DC)-3') | polymer | 11 | 3293.2 | 1 | synthetic construct | ||
3 | T | DNA (5'-D(P*TP*CP*TP*TP*GP*AP*AP*CP*GP*CP*GP*GP*TP*TP*A)-3') | polymer | 15 | 4600.0 | 1 | synthetic construct | ||
4 | A | 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE | non-polymer | 491.2 | 1 | Chemie (DTP) | |||
5 | A | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
6 | A | FE (III) ION | non-polymer | 55.8 | 1 | Chemie (FE) | |||
7 | water | water | 18.0 | 15 | Chemie (HOH) |
Sequence modifications
A: 1 - 1186 (UniProt: P21951)
PDB | External Database | Details |
---|---|---|
Gly -4 | - | expression tag |
Gly -3 | - | expression tag |
Asp -2 | - | expression tag |
Pro -1 | - | expression tag |
His 0 | - | expression tag |
Arg 301 | Pro 301 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 145128.8 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 627.2 | |
All* | Total formula weight | 145756.0 |