5Z0M

Crystal structure of copper-bound H63F-mutated tyrosinase from Streptomyces castaneoglobisporus in complex with the caddie protein obtained by soaking in the hydroxylamine-containing solution for 12 h at 298 K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Tyrosinase	polymer	281	32098.6	1	UniProt (Q83WS2) Pfam (PF00264) In PDB	Streptomyces castaneoglobisporus
2	B	MelC	polymer	134	14105.6	1	UniProt (Q83WS1) Pfam (PF06236) In PDB	Streptomyces castaneoglobisporus	caddie protein
3	A, B	COPPER (II) ION	non-polymer		63.5	3	Chemie (CU)
4	A, B	NITRATE ION	non-polymer		62.0	6	Chemie (NO3)
5		water	water		18.0	341	Chemie (HOH)

Sequence modifications

A: 1 - 273 (UniProt: Q83WS2)

PDB	External Database	Details
Phe 63	His 63	engineered mutation
Ser 123	Phe 123	conflict
Leu 274	-	expression tag
Glu 275	-	expression tag
His 276	-	expression tag
His 277	-	expression tag
His 278	-	expression tag
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag

B: 1 - 126 (UniProt: Q83WS1)

PDB	External Database	Details
Dah 98	Tyr 98	see sequence details
Leu 127	-	expression tag
Glu 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	46204.2
Non-Polymers*	Number of molecules	9
	Total formula weight	562.7
All*	Total formula weight	46766.9

*Water molecules are not included.