5X2G
Crystal structure of Campylobacter jejuni Cas9 in complex with sgRNA and target DNA (AGAAACC PAM)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR-associated endonuclease Cas9 | polymer | 835 | 96800.2 | 1 | UniProt (Q0P897) Pfam (PF18541) In PDB | Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) | |
2 | B | sgRNA | polymer | 93 | 30023.8 | 1 | Campylobacter jejuni | ||
3 | C | Target DNA strand | polymer | 28 | 8492.5 | 1 | synthetic construct | ||
4 | D | Non-target DNA strand | polymer | 8 | 2444.6 | 1 | synthetic construct | ||
5 | B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
6 | water | water | 18.0 | 319 | Chemie (HOH) |
Sequence modifications
A: 1 - 480 (UniProt: Q0P897)
A: 642 - 984 (UniProt: Q0P897)
PDB | External Database | Details |
---|---|---|
Ser -5 | - | expression tag |
Ser -4 | - | expression tag |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Gly -1 | - | expression tag |
His 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly 481 | - | linker |
Gly 637 | - | linker |
Gly 638 | - | linker |
Ser 639 | - | linker |
Gly 640 | - | linker |
Gly 641 | - | linker |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 137761.1 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 62.1 | |
All* | Total formula weight | 137823.2 |