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5X2G

Crystal structure of Campylobacter jejuni Cas9 in complex with sgRNA and target DNA (AGAAACC PAM)

Summary for 5X2G
Entry DOI10.2210/pdb5x2g/pdb
Related5X2H
DescriptorCRISPR-associated endonuclease Cas9, sgRNA, Target DNA strand, ... (6 entities in total)
Functional Keywordscrispr-cas9, rna, dna, complex, nuclease, hydrolase-rna-dna complex, hydrolase/rna/dna
Biological sourceCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
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Total number of polymer chains4
Total formula weight137823.20
Authors
Yamada, M.,Watanabe, Y.,Hirano, H.,Nakane, T.,Ishitani, R.,Nishimasu, H.,Nureki, O. (deposition date: 2017-01-31, release date: 2017-03-29, Last modification date: 2024-03-27)
Primary citationYamada, M.,Watanabe, Y.,Gootenberg, J.S.,Hirano, H.,Ran, F.A.,Nakane, T.,Ishitani, R.,Zhang, F.,Nishimasu, H.,Nureki, O.
Crystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems
Mol. Cell, 65:1109-1121.e3, 2017
Cited by
PubMed: 28306506
DOI: 10.1016/j.molcel.2017.02.007
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.4 Å)
Structure validation

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