5VI4

IL-33/ST2/IL-1RAcP ternary complex structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Interleukin-33	polymer	158	17519.3	2	UniProt (Q8BVZ5) Pfam (PF15095) In PDB	Mus musculus (Mouse)	IL-33
2	B, E	Interleukin-1 receptor-like 1	polymer	309	35509.0	2	UniProt (P14719) Pfam (PF00047) In PDB	Mus musculus (Mouse)	Interleukin-33 receptor alpha chain,Lymphocyte antigen 84,Protein ST2,Protein T1
3	C, F	Interleukin-1 receptor accessory protein	polymer	339	39470.6	2	UniProt (Q61730) Pfam (PF18452) Pfam (PF13895) Pfam (PF13927) In PDB	Mus musculus (Mouse)	IL-1RAcP,Interleukin-33 receptot beta chain
4	B, E	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)

Sequence modifications

A, D: 109 - 266 (UniProt: Q8BVZ5)

PDB	External Database	Details
Ser 139	Cys 139	engineered mutation
Ser 165	Cys 165	engineered mutation
Ser 231	Cys 231	engineered mutation
Ser 256	Cys 256	engineered mutation

B, E: 26 - 326 (UniProt: P14719)

PDB	External Database	Details
Glu 24	-	expression tag
Thr 25	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag

C, F: 21 - 350 (UniProt: Q61730)

PDB	External Database	Details
Glu 18	-	expression tag
Thr 19	-	expression tag
Gly 20	-	expression tag
Gln 107	Asn 107	engineered mutation
Gln 111	Asn 111	engineered mutation
Gln 196	Asn 196	engineered mutation
Gln 209	Asn 209	engineered mutation
His 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	184997.8
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	185661.5

*Water molecules are not included.