5VI4
IL-33/ST2/IL-1RAcP ternary complex structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03322 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 106.314, 107.332, 176.036 |
Unit cell angles | 90.00, 107.27, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.681 | 29.680 | 2.890 |
High resolution limit [Å] | 2.790 | 10.810 | 2.790 |
Rmerge | 0.108 | 0.025 | 1.130 |
Rmeas | 0.117 | 0.027 | 1.222 |
Rpim | 0.044 | 0.011 | 0.460 |
Number of reflections | 46553 | ||
<I/σ(I)> | 15 | ||
Completeness [%] | 99.0 | 95 | 93.9 |
Redundancy | 6.9 | 6.5 | 6.8 |
CC(1/2) | 0.998 | 0.999 | 0.665 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 16% PEG 3350, 0.2 M NaMalonate, pH 6, 0.2 M NaThiocyanate |