5MHF

Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Mannosyl-oligosaccharide glucosidase	polymer	795	88820.7	4	UniProt (Q80UM7) Pfam (PF16923) Pfam (PF03200) In PDB	Mus musculus (Mouse)	Glucosidase 1,Glycoprotein-processing glucosidase I
2	E, F	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
3	G, H	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
4	D, A, B, C	SULFATE ION	non-polymer		96.1	12	Chemie (SO4)
5	D, A, B, C	N-9'-methoxynonyl-1-deoxynojirimycin	non-polymer		319.4	4	Chemie (6A9)
6	D, A, B, C	2,5,8,11,14,17,20,23-OCTAOXAPENTACOSAN-25-OL	non-polymer		384.5	6	Chemie (7PG)
7		water	water		18.0	1387	Chemie (HOH)

Sequence modifications

A, B, C, D: 59 - 834 (UniProt: Q80UM7)

PDB	External Database	Details
Glu 56	-	expression tag
Thr 57	-	expression tag
Gly 58	-	expression tag
Ala 835	-	expression tag
Ser 836	-	expression tag
Trp 837	-	expression tag
Ser 838	-	expression tag
His 839	-	expression tag
Pro 840	-	expression tag
Gln 841	-	expression tag
Phe 842	-	expression tag
Glu 843	-	expression tag
Lys 844	-	expression tag
His 845	-	expression tag
His 846	-	expression tag
His 847	-	expression tag
His 848	-	expression tag
His 849	-	expression tag
His 850	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	355282.6
Branched	Number of molecules	4
	Total formula weight	2021.9
Non-Polymers*	Number of molecules	22
	Total formula weight	4737.3
All*	Total formula weight	362041.8

*Water molecules are not included.