5M0I

Crystal structure of the nuclear complex with She2p and the ASH1 mRNA E3-localization element

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	D, C, B, A	SWI5-dependent HO expression protein 2	polymer	246	28037.7	4	UniProt (B3LQW9) Pfam (PF11435) UniProt (by SIFTS) (P36068) In PDB	Saccharomyces cerevisiae
2	E, F	ASH1-E3 element, RNA (28-MER)	polymer	28	8994.4	2		Saccharomyces cerevisiae
3	H, J, I	SWI5-dependent HO expression protein 3	polymer	24	2522.7	3	UniProt (P38272) In PDB	Saccharomyces cerevisiae S288c
4	D, C, B, A, E	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
5	F, D, C, B, A	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
6	E, F, D, B	ACETATE ION	non-polymer		59.0	6	Chemie (ACT)
7		water	water		18.0	333	Chemie (HOH)

Sequence modifications

D, C, B, A: 6 - 246 (UniProt: B3LQW9)

PDB	External Database	Details
Gly 1	-	expression tag
Pro 2	-	expression tag
Leu 3	-	expression tag
Gly 4	-	expression tag
Ser 5	-	expression tag
Ser 14	Cys 14	engineered mutation
Ser 68	Cys 68	engineered mutation
Ser 106	Cys 106	engineered mutation
Ser 180	Cys 180	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	137708.1
Non-Polymers*	Number of molecules	23
	Total formula weight	1182.8
All*	Total formula weight	138890.9

*Water molecules are not included.