5M0I
Crystal structure of the nuclear complex with She2p and the ASH1 mRNA E3-localization element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0716 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 220.520, 58.340, 146.010 |
| Unit cell angles | 90.00, 126.98, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.410 |
| R-factor | 0.19175 |
| Rwork | 0.189 |
| R-free | 0.23458 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xly |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.881 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.470 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.068 | 0.438 |
| Number of reflections | 49733 | |
| <I/σ(I)> | 10.37 | 2.13 |
| Completeness [%] | 90.1 | 91 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM Bis Tris propane, pH 6.5, 200 mM NaF, 20 % (w/v) PEG 3350, |
