5KKL

Structure of ctPRC2 in complex with H3K27me3 and H3K27M

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	putative polycomb protein Eed	polymer	605	66021.4	1	UniProt (G0S8H7) Pfam (PF00400) In PDB	Chaetomium thermophilum
2	B	Putative uncharacterized protein,Histone H3.1 peptide,Zinc finger domain-containing protein	polymer	938	106890.6	1	UniProt (G0SDW4) UniProt (P68431) UniProt (G0RYC6) Pfam (PF18601) Pfam (PF18600) Pfam (PF21509) Pfam (PF00856) Pfam (PF09733) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
3	D	ALA-ALA-ARG-M3L-SER-ALA-PRO-ALA	polymer	11	1146.4	1	UniProt (by SIFTS) (P68431) In PDB	Homo sapiens
4	B	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
5	B	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)

Sequence modifications

A: 1 - 565 (UniProt: G0S8H7)

PDB	External Database	Details
Met -39	-	expression tag
Ala -38	-	expression tag
Ser -37	-	expression tag
Ala -36	-	expression tag
Trp -35	-	expression tag
Ser -34	-	expression tag
His -33	-	expression tag
Pro -32	-	expression tag
Gln -31	-	expression tag
Phe -30	-	expression tag
Glu -29	-	expression tag
Lys -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

B: 191 - 2013 (UniProt: G0SDW4)

PDB	External Database	Details
Ser 182	-	expression tag
Asn 183	-	expression tag
His 184	-	expression tag
His 185	-	expression tag
His 186	-	expression tag
His 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag
Ala 190	-	expression tag
Leu 2014	-	linker
Val 2015	-	linker
Pro 2016	-	linker
Ala 2017	-	linker
Gly 2018	-	linker
Ser 2019	-	linker
Glu 2020	-	linker
Val 2021	-	linker

B: 2022 - 2030 (UniProt: P68431)

PDB	External Database	Details
Met 2027	Lys 28	engineered mutation

B: 2031 - 2691 (UniProt: G0RYC6)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	174058.3
Non-Polymers*	Number of molecules	9
	Total formula weight	921.7
All*	Total formula weight	174980.0

*Water molecules are not included.