5EGH

Structure of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase in complex with phosphocholine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ectonucleotide pyrophosphatase/phosphodiesterase family member 6	polymer	429	49598.7	2	UniProt (Q8BGN3) Pfam (PF01663) In PDB	Mus musculus (Mouse)	NPP-6,Choline-specific glycerophosphodiester phosphodiesterase,Glycerophosphocholine cholinephosphodiesterase,GPC-Cpde
2	C, E, G	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
3	D, F	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	2	In PDB GlyTouCan (G28454KX)
4	A, B	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	A, B	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
6	A, B	PHOSPHOCHOLINE	non-polymer		184.2	2	Chemie (PC)
7	B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
8		water	water		18.0	528	Chemie (HOH)

Sequence modifications

A, B: 1 - 421 (UniProt: Q8BGN3)

PDB	External Database	Details
Ala 393	Cys 393	engineered mutation
Ser 412	Cys 412	engineered mutation
Ser 422	-	expression tag
Arg 423	-	expression tag
Glu 424	-	expression tag
Asn 425	-	expression tag
Leu 426	-	expression tag
Tyr 427	-	expression tag
Phe 428	-	expression tag
Gln 429	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	99197.3
Branched	Number of molecules	5
	Total formula weight	2414.3
Non-Polymers*	Number of molecules	12
	Total formula weight	1161.5
All*	Total formula weight	102773.1

*Water molecules are not included.