5EGH
Structure of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase in complex with phosphocholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.278 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.620, 68.849, 69.761 |
| Unit cell angles | 60.59, 86.99, 68.10 |
Refinement procedure
| Resolution | 45.634 - 1.803 |
| R-factor | 0.1766 |
| Rwork | 0.175 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.895 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 81806 |
| <I/σ(I)> | 12.8 |
| Completeness [%] | 94.0 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | sodium acetate, ammonium chloride, PEG 6000, zinc sulfate |
