4YLP
E. coli Transcription Initiation Complex - 16-bp spacer and 5-nt RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, G, H, M... | DNA-directed RNA polymerase subunit alpha | polymer | 242 | 26899.6 | 6 | UniProt (A7ZSI4) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) In PDB | Escherichia coli | RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha |
| 2 | C, I, O | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 3 | UniProt (A7ZUK1) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) In PDB | Escherichia coli | RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta |
| 3 | D, J, P | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 3 | UniProt (A7ZUK2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB | Escherichia coli | RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta' |
| 4 | E, K, Q | DNA-directed RNA polymerase subunit omega | polymer | 90 | 10118.4 | 3 | UniProt (A7ZTK1) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB | Escherichia coli | RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega |
| 5 | F, L, R | RNA polymerase sigma factor RpoD | polymer | 628 | 72206.3 | 3 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Escherichia coli | Sigma-70 |
| 6 | 1, 4, 7 | NT strand DNA (49-MER) | polymer | 49 | 15024.6 | 3 | synthetic construct | ||
| 7 | 2, 5, 8 | T strand DNA (49-MER) | polymer | 49 | 15233.8 | 3 | synthetic construct | ||
| 8 | 3, 6, 9 | RNA (5'-R(*(GTP))-R(P*AP*GP*UP*C)-3') | polymer | 5 | 1746.0 | 3 | synthetic construct | ||
| 9 | D, J, P | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) | |||
| 10 | D, J, P | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) |
Sequence modifications
A, B, G, H, M, N: 1 - 235 (UniProt: A7ZSI4)
F, L, R: 1 - 613 (UniProt: P00579)
| PDB | External Database | Details |
|---|---|---|
| Ala -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -14 | - | expression tag |
| Arg -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| Thr -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Thr 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 27 |
| Total formula weight | 1422947.3 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 465.4 | |
| All* | Total formula weight | 1423412.7 |
