4YLP
E. coli Transcription Initiation Complex - 16-bp spacer and 5-nt RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 237.674, 204.988, 248.838 |
Unit cell angles | 90.00, 116.86, 90.00 |
Refinement procedure
Resolution | 39.980 - 5.500 |
R-factor | 0.23488 |
Rwork | 0.231 |
R-free | 0.31302 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.547 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 5.500 |
Number of reflections | 69757 |
<I/σ(I)> | 12.3 |
Completeness [%] | 99.9 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 7% PEG3350, 150 mM magnesium chloride, 100 mM HEPES sodium, pH 7.0 |