4KHW

Ternary complex of RB69 mutant L415F with ribonucleotide at -2 position

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase	polymer	903	104689.2	1	UniProt (Q38087) Pfam (PF03104) Pfam (PF00136) In PDB	Enterobacteria phage RB69	Gp43
2	T	DNA/RNA (5'-D(*AP*CP*AP*G)-R(P*G)-D(P*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3')	polymer	18	5566.6	1
3	P	DNA (5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*CP*C)-3')	polymer	14	4256.8	1
4	A	THYMIDINE-5'-TRIPHOSPHATE	non-polymer		482.2	1	Chemie (TTP)
5	A	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
6	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
7		water	water		18.0	534	Chemie (HOH)

Sequence modifications

A: 1 - 903 (UniProt: Q38087)

PDB	External Database	Details
Ala 222	Asp 222	engineered mutation
Ala 327	Asp 327	engineered mutation
Phe 415	Leu 415	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	114512.5
Non-Polymers*	Number of molecules	11
	Total formula weight	848.8
All*	Total formula weight	115361.3

*Water molecules are not included.