4FK3

B-Raf Kinase V600E Oncogenic Mutant in Complex with PLX3203

Replaces:  3C4D

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Serine/threonine-protein kinase B-raf	polymer	292	33325.1	2	UniProt (P15056) Pfam (PF07714) In PDB	Homo sapiens (human)	Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1
2	A	N-{2,4-difluoro-3-[(5-pyridin-3-yl-1H-pyrrolo[2,3-b]pyridin-3-yl)carbonyl]phenyl}ethanesulfonamide	non-polymer		442.4	1	Chemie (325)
3		water	water		18.0	29	Chemie (HOH)

Sequence modifications

A, B: 444 - 723 (UniProt: P15056)

PDB	External Database	Details
Met 432	-	expression tag
Lys 433	-	expression tag
Lys 434	-	expression tag
Gly 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag
His 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
Gly 442	-	expression tag
Ser 443	-	expression tag
Ala 543	Ile 542	engineered mutation
Ser 544	Ile 543	engineered mutation
Lys 551	Ile 550	engineered mutation
Arg 562	Gln 561	engineered mutation
Asn 588	Leu 587	engineered mutation
Glu 600	Val 599	engineered mutation
Ser 630	Lys 629	engineered mutation
Glu 667	Phe 666	engineered mutation
Ser 673	Tyr 672	engineered mutation
Arg 688	Ala 687	engineered mutation
Ser 706	Leu 705	engineered mutation
Arg 709	Gln 708	engineered mutation
Glu 713	Ser 712	engineered mutation
Glu 716	Leu 715	engineered mutation
Glu 720	Ser 719	engineered mutation
Ser 722	Pro 721	engineered mutation
Gly 723	Lys 722	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66650.3
Non-Polymers*	Number of molecules	1
	Total formula weight	442.4
All*	Total formula weight	67092.7

*Water molecules are not included.