4EXK

A chimera protein containing MBP fused to the C-terminal domain of the uncharacterized protein STM14_2015 from Salmonella enterica

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Maltose-binding periplasmic protein, uncharacterized protein chimera	polymer	487	53007.9	1	UniProt (P0AEX9) UniProt (D0ZI82) Pfam (PF01547) Pfam (PF16583) UniProt (by SIFTS) (A0A0F6B1U8) In PDB	Escherichia coli	MBP, MMBP, Maltodextrin-binding protein
2	B	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		666.6	1	In PDB BIRD (PRD_900010) GlyTouCan (G87171PZ)		alpha-maltotetraose
3	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
4		water	water		18.0	492	Chemie (HOH)

Sequence modifications

A: 0 - 366 (UniProt: P0AEX9)

PDB	External Database	Details
Ser -3	-	expression tag
Asn -2	-	expression tag
Ala -1	-	expression tag
Ala 82	Asp 108	engineered mutation
Ala 83	Lys 109	engineered mutation
Ala 172	Glu 198	engineered mutation
Ala 173	Asn 199	engineered mutation
Ala 239	Lys 265	engineered mutation
Ala 359	Glu 385	engineered mutation
Ala 362	Lys 388	engineered mutation
Ala 363	Asp 389	engineered mutation

A: 370 - 473 (UniProt: D0ZI82)

PDB	External Database	Details
Asn 367	-	linker
Ala 368	-	linker
Ala 369	-	linker
Ala 370	Ser 173	engineered mutation
Ser 474	-	expression tag
Leu 475	-	expression tag
Ser 476	-	expression tag
Ser 477	-	expression tag
Val 478	-	expression tag
Lys 479	-	expression tag
Asn 480	-	expression tag
Asn 481	-	expression tag
Ile 482	-	expression tag
Gly 483	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	53007.9
Branched	Number of molecules	1
	Total formula weight	666.6
Non-Polymers*	Number of molecules	1
	Total formula weight	150.2
All*	Total formula weight	53824.7

*Water molecules are not included.