4EXK
A chimera protein containing MBP fused to the C-terminal domain of the uncharacterized protein STM14_2015 from Salmonella enterica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.079, 64.079, 180.912 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 1.280 |
R-factor | 0.14324 |
Rwork | 0.141 |
R-free | 0.17772 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hpi |
RMSD bond length | 0.017 |
RMSD bond angle | 2.203 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.300 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.057 | |
Number of reflections | 103107 | |
<I/σ(I)> | 28.5 | |
Completeness [%] | 92.1 | 76 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.2 M Ammonium Acetate, 25 % peg 3320, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |