4AHC

Crystal Structure of an Evolved Replicating DNA Polymerase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA POLYMERASE	polymer	775	90113.2	2	UniProt (P61875) Pfam (PF03104) Pfam (PF00136) In PDB	PYROCOCCUS FURIOSUS	PFU POLYMERASE
2	A, B	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
3		water	water		18.0	294	Chemie (HOH)

Sequence modifications

A, B: 1 - 775 (UniProt: P61875)

PDB	External Database	Details
Gln 93	Val 93	engineered mutation
Ala 141	Asp 141	engineered mutation
Ala 143	Glu 143	engineered mutation
Ile 337	Val 337	engineered mutation
Asp 399	Glu 399	engineered mutation
Asp 400	Asn 400	engineered mutation
Ile 407	Arg 407	engineered mutation
His 546	Tyr 546	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	180226.3
Non-Polymers*	Number of molecules	4
	Total formula weight	368.4
All*	Total formula weight	180594.7

*Water molecules are not included.