3W5G

Crystal structure of tomato beta-galactosidase 4 in complex with galactose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-galactosidase	polymer	718	79784.9	2	UniProt (O81100) Pfam (PF01301) Pfam (PF17834) Pfam (PF21467) In PDB	Solanum lycopersicum (tomato)
2	C	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
4	A, B	beta-D-galactopyranose	non-polymer		180.2	8	Chemie (GAL)
5		water	water		18.0	198	Chemie (HOH)

Sequence modifications

A, B: 24 - 724 (UniProt: O81100)

PDB	External Database	Details
Glu 18	-	expression tag
Ala 19	-	expression tag
Glu 20	-	expression tag
Ala 21	-	expression tag
Glu 22	-	expression tag
Phe 23	-	expression tag
Ser 725	-	expression tag
Ala 726	-	expression tag
Ala 727	-	expression tag
Ala 728	-	expression tag
Ala 729	-	expression tag
Ser 730	-	expression tag
Phe 731	-	expression tag
Leu 732	-	expression tag
Glu 733	-	expression tag
Gln 734	-	expression tag
Lys 735	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	159569.9
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	11
	Total formula weight	2104.9
All*	Total formula weight	162099.2

*Water molecules are not included.