1T36
Crystal structure of E. coli carbamoyl phosphate synthetase small subunit mutant C248D complexed with uridine 5'-monophosphate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G | Carbamoyl-phosphate synthase large chain | polymer | 1073 | 117981.8 | 4 | UniProt (P00968) Pfam (PF02786) Pfam (PF02787) Pfam (PF02142) In PDB | Escherichia coli | Carbamoyl-phosphate synthetase ammonia chain |
| 2 | B, D, F, H | Carbamoyl-phosphate synthase small chain | polymer | 382 | 41493.8 | 4 | UniProt (P00907) Pfam (PF00988) Pfam (PF00117) Pfam (PF07722) UniProt (by SIFTS) (P0A6F1) In PDB | Escherichia coli | Carbamoyl-phosphate synthetase glutamine chain |
| 3 | C, E, A, G | MANGANESE (II) ION | non-polymer | 54.9 | 12 | Chemie (MN) | |||
| 4 | B, E, G, C, A... | POTASSIUM ION | non-polymer | 39.1 | 22 | Chemie (K) | |||
| 5 | E, C, A, G | PHOSPHATE ION | non-polymer | 95.0 | 5 | Chemie (PO4) | |||
| 6 | G, E, C, H, A... | CHLORIDE ION | non-polymer | 35.5 | 27 | Chemie (CL) | |||
| 7 | G, E, C, A | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 8 | Chemie (ADP) | |||
| 8 | G, E, C, A | L-ornithine | non-polymer | 132.2 | 4 | Chemie (ORN) | |||
| 9 | G, E, C, A | TETRAETHYLAMMONIUM ION | non-polymer | 130.3 | 4 | Chemie (NET) | |||
| 10 | G, E, C, A | URIDINE-5'-MONOPHOSPHATE | non-polymer | 324.2 | 4 | Chemie (U) | |||
| 11 | water | water | 18.0 | 3907 | Chemie (HOH) |
Sequence modifications
B, D, F, H: 1 - 382 (UniProt: P00907)
| PDB | External Database | Details |
|---|---|---|
| Asp 248 | Cys 248 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 637902.3 | |
| Non-Polymers* | Number of molecules | 86 |
| Total formula weight | 8715.5 | |
| All* | Total formula weight | 646617.8 |
