1T36
Crystal structure of E. coli carbamoyl phosphate synthetase small subunit mutant C248D complexed with uridine 5'-monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-11 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.65 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 152.500, 164.900, 333.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.20900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1jdb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.430 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 433843 | |
| <I/σ(I)> | 15.2 | 3.3 |
| Completeness [%] | 90.0 | 83 |
| Redundancy | 2.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 7.4 | 277 | PEG 8000, tetramethylammonium chloride, potassium chloride, manganese chloride, ornithine, ADP, HEPES, pH 7.4, batch, temperature 277K |
