8QCF

yeast cytoplasmic exosome-Ski2 complex degrading a RNA substrate

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0000176	cellular_component	nuclear exosome (RNase complex)
B	0000177	cellular_component	cytoplasmic exosome (RNase complex)
B	0000178	cellular_component	exosome (RNase complex)
B	0000288	biological_process	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
B	0000467	biological_process	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
B	0000956	biological_process	nuclear-transcribed mRNA catabolic process
B	0003674	molecular_function	molecular_function
B	0003723	molecular_function	RNA binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005730	cellular_component	nucleolus
B	0005737	cellular_component	cytoplasm
B	0006364	biological_process	rRNA processing
B	0006396	biological_process	RNA processing
B	0006401	biological_process	RNA catabolic process
B	0016075	biological_process	rRNA catabolic process
B	0034473	biological_process	U1 snRNA 3'-end processing
B	0034475	biological_process	U4 snRNA 3'-end processing
B	0034476	biological_process	U5 snRNA 3'-end processing
B	0035925	molecular_function	mRNA 3'-UTR AU-rich region binding
B	0071028	biological_process	nuclear mRNA surveillance
B	0071035	biological_process	nuclear polyadenylation-dependent rRNA catabolic process
B	0071038	biological_process	TRAMP-dependent tRNA surveillance pathway
C	0000176	cellular_component	nuclear exosome (RNase complex)
C	0000177	cellular_component	cytoplasmic exosome (RNase complex)
C	0000178	cellular_component	exosome (RNase complex)
C	0000467	biological_process	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
C	0000956	biological_process	nuclear-transcribed mRNA catabolic process
C	0003674	molecular_function	molecular_function
C	0003723	molecular_function	RNA binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005730	cellular_component	nucleolus
C	0005737	cellular_component	cytoplasm
C	0006364	biological_process	rRNA processing
C	0006396	biological_process	RNA processing
C	0006401	biological_process	RNA catabolic process
C	0016075	biological_process	rRNA catabolic process
C	0034473	biological_process	U1 snRNA 3'-end processing
C	0034475	biological_process	U4 snRNA 3'-end processing
C	0034476	biological_process	U5 snRNA 3'-end processing
C	0070478	biological_process	nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay
C	0071028	biological_process	nuclear mRNA surveillance
C	0071038	biological_process	TRAMP-dependent tRNA surveillance pathway
C	0071039	biological_process	nuclear polyadenylation-dependent CUT catabolic process
C	0071051	biological_process	poly(A)-dependent snoRNA 3'-end processing
D	0000176	cellular_component	nuclear exosome (RNase complex)
D	0000177	cellular_component	cytoplasmic exosome (RNase complex)
D	0000178	cellular_component	exosome (RNase complex)
D	0000288	biological_process	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
D	0000467	biological_process	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
D	0000956	biological_process	nuclear-transcribed mRNA catabolic process
D	0003674	molecular_function	molecular_function
D	0003723	molecular_function	RNA binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005730	cellular_component	nucleolus
D	0005737	cellular_component	cytoplasm
D	0006364	biological_process	rRNA processing
D	0006396	biological_process	RNA processing
D	0006397	biological_process	mRNA processing
D	0006401	biological_process	RNA catabolic process
D	0006402	biological_process	mRNA catabolic process
D	0016072	biological_process	rRNA metabolic process
D	0016075	biological_process	rRNA catabolic process
D	0034473	biological_process	U1 snRNA 3'-end processing
D	0034475	biological_process	U4 snRNA 3'-end processing
D	0034476	biological_process	U5 snRNA 3'-end processing
D	0035925	molecular_function	mRNA 3'-UTR AU-rich region binding
D	0071028	biological_process	nuclear mRNA surveillance
D	0071035	biological_process	nuclear polyadenylation-dependent rRNA catabolic process
D	0071038	biological_process	TRAMP-dependent tRNA surveillance pathway
D	0071042	biological_process	nuclear polyadenylation-dependent mRNA catabolic process
E	0000176	cellular_component	nuclear exosome (RNase complex)
E	0000177	cellular_component	cytoplasmic exosome (RNase complex)
E	0000178	cellular_component	exosome (RNase complex)
E	0000467	biological_process	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
E	0005634	cellular_component	nucleus
E	0005730	cellular_component	nucleolus
E	0006364	biological_process	rRNA processing
E	0016075	biological_process	rRNA catabolic process
E	0034475	biological_process	U4 snRNA 3'-end processing
E	0071028	biological_process	nuclear mRNA surveillance
E	0071038	biological_process	TRAMP-dependent tRNA surveillance pathway
E	0071051	biological_process	poly(A)-dependent snoRNA 3'-end processing
F	0000176	cellular_component	nuclear exosome (RNase complex)
F	0000177	cellular_component	cytoplasmic exosome (RNase complex)
F	0000178	cellular_component	exosome (RNase complex)
F	0000288	biological_process	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
F	0000467	biological_process	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
F	0000956	biological_process	nuclear-transcribed mRNA catabolic process
F	0003674	molecular_function	molecular_function
F	0003723	molecular_function	RNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005730	cellular_component	nucleolus
F	0005737	cellular_component	cytoplasm
F	0006364	biological_process	rRNA processing
F	0006396	biological_process	RNA processing
F	0006401	biological_process	RNA catabolic process
F	0016075	biological_process	rRNA catabolic process
F	0034473	biological_process	U1 snRNA 3'-end processing
F	0034475	biological_process	U4 snRNA 3'-end processing
F	0034476	biological_process	U5 snRNA 3'-end processing
F	0035925	molecular_function	mRNA 3'-UTR AU-rich region binding
F	0071028	biological_process	nuclear mRNA surveillance
F	0071035	biological_process	nuclear polyadenylation-dependent rRNA catabolic process
F	0071038	biological_process	TRAMP-dependent tRNA surveillance pathway
G	0000176	cellular_component	nuclear exosome (RNase complex)
G	0000177	cellular_component	cytoplasmic exosome (RNase complex)
G	0000178	cellular_component	exosome (RNase complex)
G	0000467	biological_process	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
G	0000956	biological_process	nuclear-transcribed mRNA catabolic process
G	0003674	molecular_function	molecular_function
G	0003723	molecular_function	RNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005730	cellular_component	nucleolus
G	0005737	cellular_component	cytoplasm
G	0006364	biological_process	rRNA processing
G	0006396	biological_process	RNA processing
G	0006401	biological_process	RNA catabolic process
G	0016075	biological_process	rRNA catabolic process
G	0034475	biological_process	U4 snRNA 3'-end processing
G	0071028	biological_process	nuclear mRNA surveillance
G	0071035	biological_process	nuclear polyadenylation-dependent rRNA catabolic process
G	0071038	biological_process	TRAMP-dependent tRNA surveillance pathway
G	0071039	biological_process	nuclear polyadenylation-dependent CUT catabolic process
G	0071051	biological_process	poly(A)-dependent snoRNA 3'-end processing
H	0000176	cellular_component	nuclear exosome (RNase complex)
H	0000177	cellular_component	cytoplasmic exosome (RNase complex)
H	0000178	cellular_component	exosome (RNase complex)
H	0000288	biological_process	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
H	0000467	biological_process	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
H	0000956	biological_process	nuclear-transcribed mRNA catabolic process
H	0003723	molecular_function	RNA binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005730	cellular_component	nucleolus
H	0005737	cellular_component	cytoplasm
H	0006364	biological_process	rRNA processing
H	0006396	biological_process	RNA processing
H	0006401	biological_process	RNA catabolic process
H	0030145	molecular_function	manganese ion binding
H	0034475	biological_process	U4 snRNA 3'-end processing
H	0043229	cellular_component	intracellular organelle
H	0071034	biological_process	CUT catabolic process
H	0071035	biological_process	nuclear polyadenylation-dependent rRNA catabolic process
H	0071038	biological_process	TRAMP-dependent tRNA surveillance pathway
H	0071051	biological_process	poly(A)-dependent snoRNA 3'-end processing
I	0000176	cellular_component	nuclear exosome (RNase complex)
I	0000177	cellular_component	cytoplasmic exosome (RNase complex)
I	0000178	cellular_component	exosome (RNase complex)
I	0000288	biological_process	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
I	0000467	biological_process	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
I	0000956	biological_process	nuclear-transcribed mRNA catabolic process
I	0003674	molecular_function	molecular_function
I	0003723	molecular_function	RNA binding
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005730	cellular_component	nucleolus
I	0005737	cellular_component	cytoplasm
I	0006364	biological_process	rRNA processing
I	0006396	biological_process	RNA processing
I	0006401	biological_process	RNA catabolic process
I	0034475	biological_process	U4 snRNA 3'-end processing
I	0071028	biological_process	nuclear mRNA surveillance
I	0071034	biological_process	CUT catabolic process
I	0071035	biological_process	nuclear polyadenylation-dependent rRNA catabolic process
I	0071038	biological_process	TRAMP-dependent tRNA surveillance pathway
I	0071051	biological_process	poly(A)-dependent snoRNA 3'-end processing
J	0000176	cellular_component	nuclear exosome (RNase complex)
J	0000177	cellular_component	cytoplasmic exosome (RNase complex)
J	0000178	cellular_component	exosome (RNase complex)
J	0000467	biological_process	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
J	0000956	biological_process	nuclear-transcribed mRNA catabolic process
J	0003674	molecular_function	molecular_function
J	0003723	molecular_function	RNA binding
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005730	cellular_component	nucleolus
J	0005737	cellular_component	cytoplasm
J	0006364	biological_process	rRNA processing
J	0006396	biological_process	RNA processing
J	0006397	biological_process	mRNA processing
J	0006401	biological_process	RNA catabolic process
J	0070481	biological_process	nuclear-transcribed mRNA catabolic process, non-stop decay
J	0071035	biological_process	nuclear polyadenylation-dependent rRNA catabolic process
J	0071038	biological_process	TRAMP-dependent tRNA surveillance pathway
K	0000049	molecular_function	tRNA binding
K	0000175	molecular_function	3'-5'-RNA exonuclease activity
K	0000176	cellular_component	nuclear exosome (RNase complex)
K	0000177	cellular_component	cytoplasmic exosome (RNase complex)
K	0000178	cellular_component	exosome (RNase complex)
K	0000467	biological_process	exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
K	0003676	molecular_function	nucleic acid binding
K	0003723	molecular_function	RNA binding
K	0004519	molecular_function	endonuclease activity
K	0004521	molecular_function	RNA endonuclease activity
K	0004527	molecular_function	exonuclease activity
K	0004540	molecular_function	RNA nuclease activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005730	cellular_component	nucleolus
K	0005737	cellular_component	cytoplasm
K	0005739	cellular_component	mitochondrion
K	0006364	biological_process	rRNA processing
K	0006396	biological_process	RNA processing
K	0006397	biological_process	mRNA processing
K	0006401	biological_process	RNA catabolic process
K	0016075	biological_process	rRNA catabolic process
K	0043628	biological_process	regulatory ncRNA 3'-end processing
K	0070651	biological_process	nonfunctional rRNA decay
K	0071028	biological_process	nuclear mRNA surveillance
K	0071031	biological_process	nuclear mRNA surveillance of mRNA 3'-end processing
K	0071035	biological_process	nuclear polyadenylation-dependent rRNA catabolic process
K	0071038	biological_process	TRAMP-dependent tRNA surveillance pathway
K	0071039	biological_process	nuclear polyadenylation-dependent CUT catabolic process
M	0003676	molecular_function	nucleic acid binding
M	0003723	molecular_function	RNA binding
M	0003724	molecular_function	RNA helicase activity
M	0005524	molecular_function	ATP binding
M	0006401	biological_process	RNA catabolic process

Functional Information from PROSITE/UniProt

site_id	PS01175
Number of Residues	25
Details	RIBONUCLEASE_II Ribonuclease II family signature. HYGLavdi.YThFTSPIRRYcDvvaH

Chain	Residue	Details
K	HIS831-HIS855

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17287358, ECO:0007744|PubMed:18407956

Chain	Residue	Details
J	SER94
L	SER90

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198

Chain	Residue	Details
I	SER28

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956

Chain	Residue	Details
I	SER268

---