8F6C

E. coli cytochrome bo3 ubiquinol oxidase dimer

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004129	molecular_function	cytochrome-c oxidase activity
A	0005507	molecular_function	copper ion binding
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0009055	molecular_function	electron transfer activity
A	0009060	biological_process	aerobic respiration
A	0009319	cellular_component	cytochrome o ubiquinol oxidase complex
A	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
A	0015078	molecular_function	proton transmembrane transporter activity
A	0015453	molecular_function	oxidoreduction-driven active transmembrane transporter activity
A	0015990	biological_process	electron transport coupled proton transport
A	0016020	cellular_component	membrane
A	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
A	0019646	biological_process	aerobic electron transport chain
A	0020037	molecular_function	heme binding
A	0022904	biological_process	respiratory electron transport chain
A	0046872	molecular_function	metal ion binding
A	0048039	molecular_function	ubiquinone binding
A	0070469	cellular_component	respirasome
A	1902600	biological_process	proton transmembrane transport
B	0004129	molecular_function	cytochrome-c oxidase activity
B	0005507	molecular_function	copper ion binding
B	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
B	0016020	cellular_component	membrane
B	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
B	0022900	biological_process	electron transport chain
C	0004129	molecular_function	cytochrome-c oxidase activity
C	0005515	molecular_function	protein binding
C	0005886	cellular_component	plasma membrane
C	0009055	molecular_function	electron transfer activity
C	0009060	biological_process	aerobic respiration
C	0009319	cellular_component	cytochrome o ubiquinol oxidase complex
C	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
C	0015078	molecular_function	proton transmembrane transporter activity
C	0015453	molecular_function	oxidoreduction-driven active transmembrane transporter activity
C	0015990	biological_process	electron transport coupled proton transport
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0019646	biological_process	aerobic electron transport chain
C	0022904	biological_process	respiratory electron transport chain
D	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
D	0015990	biological_process	electron transport coupled proton transport
D	0016020	cellular_component	membrane
E	0004129	molecular_function	cytochrome-c oxidase activity
E	0005507	molecular_function	copper ion binding
E	0005515	molecular_function	protein binding
E	0005886	cellular_component	plasma membrane
E	0009055	molecular_function	electron transfer activity
E	0009060	biological_process	aerobic respiration
E	0009319	cellular_component	cytochrome o ubiquinol oxidase complex
E	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
E	0015078	molecular_function	proton transmembrane transporter activity
E	0015453	molecular_function	oxidoreduction-driven active transmembrane transporter activity
E	0015990	biological_process	electron transport coupled proton transport
E	0016020	cellular_component	membrane
E	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
E	0019646	biological_process	aerobic electron transport chain
E	0020037	molecular_function	heme binding
E	0022904	biological_process	respiratory electron transport chain
E	0046872	molecular_function	metal ion binding
E	0048039	molecular_function	ubiquinone binding
E	0070469	cellular_component	respirasome
E	1902600	biological_process	proton transmembrane transport
F	0004129	molecular_function	cytochrome-c oxidase activity
F	0005507	molecular_function	copper ion binding
F	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
F	0016020	cellular_component	membrane
F	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
F	0022900	biological_process	electron transport chain
G	0004129	molecular_function	cytochrome-c oxidase activity
G	0005515	molecular_function	protein binding
G	0005886	cellular_component	plasma membrane
G	0009055	molecular_function	electron transfer activity
G	0009060	biological_process	aerobic respiration
G	0009319	cellular_component	cytochrome o ubiquinol oxidase complex
G	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
G	0015078	molecular_function	proton transmembrane transporter activity
G	0015453	molecular_function	oxidoreduction-driven active transmembrane transporter activity
G	0015990	biological_process	electron transport coupled proton transport
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0019646	biological_process	aerobic electron transport chain
G	0022904	biological_process	respiratory electron transport chain
H	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
H	0015990	biological_process	electron transport coupled proton transport
H	0016020	cellular_component	membrane

Functional Information from PROSITE/UniProt

site_id	PS00077
Number of Residues	55
Details	COX1_CUB Heme-copper oxidase catalytic subunit, copper B binding region signature. WAWGHPeVyililpvfgvfseiaatfsrkrlfgytslvwatvcitvlsfivwl..HH

Chain	Residue	Details
A	TRP280-HIS334

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	108
Details	TRANSMEM: Helical => ECO:0000305

Chain	Residue	Details
D	TYR18-VAL36
D	ILE46-CYS64
D	PHE81-MET99
H	TYR18-VAL36
H	ILE46-CYS64
H	PHE81-MET99
G	PRO67-MET85
G	LEU102-PHE120
G	VAL143-MET161
G	HIS185-ALA203
E	VAL162-GLY184
E	THR261-GLY270
E	SER327-ALA340
E	LEU410-ASN412
E	GLY478-PRO489
E	ILE607-LEU613

---

site_id	SWS_FT_FI2
Number of Residues	34
Details	TOPO_DOM: Periplasmic => ECO:0000305

Chain	Residue	Details
D	MET37-VAL45
D	TRP100-HIS109
H	MET37-VAL45
H	TRP100-HIS109
G	HIS121-LEU142
G	MET204

---

site_id	SWS_FT_FI3
Number of Residues	30
Details	TOPO_DOM: Cytoplasmic => ECO:0000305

Chain	Residue	Details
D	PHE65-ALA80
H	PHE65-ALA80
G	ALA86-TRP101
G	VAL162-TRP184
A	MET370-PHE377
A	PHE446-LEU448
A	ASP522-LYS587
E	THR36-VAL52
E	ALA133-ALA137
E	ARG216-LYS224
E	LYS308-PHE311
E	MET370-PHE377
E	PHE446-LEU448
E	ASP522-LYS587

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	LIPID: S-diacylglycerol cysteine => ECO:0000255|PROSITE-ProRule:PRU00303

Chain	Residue	Details
B	CYS25
F	CYS25

---

site_id	SWS_FT_FI5
Number of Residues	72
Details	TRANSMEM: Helical; Name=III

Chain	Residue	Details
A	PRO96-GLY132
E	PRO96-GLY132

---

site_id	SWS_FT_FI6
Number of Residues	46
Details	TRANSMEM: Helical; Name=IV

Chain	Residue	Details
A	PHE138-GLY161
E	PHE138-GLY161

---

site_id	SWS_FT_FI7
Number of Residues	60
Details	TRANSMEM: Helical; Name=V

Chain	Residue	Details
A	VAL185-MET215
E	VAL185-MET215

---

site_id	SWS_FT_FI8
Number of Residues	70
Details	TRANSMEM: Helical; Name=VI

Chain	Residue	Details
A	MET225-GLY260
E	MET225-GLY260

---

site_id	SWS_FT_FI9
Number of Residues	72
Details	TRANSMEM: Helical; Name=VII

Chain	Residue	Details
A	ASN271-ARG307
E	ASN271-ARG307

---

site_id	SWS_FT_FI10
Number of Residues	28
Details	TRANSMEM: Helical; Name=VIII

Chain	Residue	Details
A	GLY312-LEU326
E	GLY312-LEU326

---

site_id	SWS_FT_FI11
Number of Residues	56
Details	TRANSMEM: Helical; Name=IX

Chain	Residue	Details
A	GLY341-THR369
E	GLY341-THR369

---

site_id	SWS_FT_FI12
Number of Residues	62
Details	TRANSMEM: Helical; Name=X

Chain	Residue	Details
A	HIS378-VAL409
E	HIS378-VAL409

---

site_id	SWS_FT_FI13
Number of Residues	64
Details	TRANSMEM: Helical; Name=XI

Chain	Residue	Details
A	SER413-GLY445
E	SER413-GLY445

---

site_id	SWS_FT_FI14
Number of Residues	56
Details	TRANSMEM: Helical; Name=XII

Chain	Residue	Details
A	ASN449-MET477
E	ASN449-MET477

---

site_id	SWS_FT_FI15
Number of Residues	62
Details	TRANSMEM: Helical; Name=XIII

Chain	Residue	Details
A	GLN490-ARG521
E	GLN490-ARG521

---

site_id	SWS_FT_FI16
Number of Residues	36
Details	TRANSMEM: Helical; Name=XIV

Chain	Residue	Details
A	ASN588-MET606
E	ASN588-MET606

---

site_id	SWS_FT_FI17
Number of Residues	36
Details	TRANSMEM: Helical; Name=XV

Chain	Residue	Details
A	ALA614-GLU632
E	ALA614-GLU632

---

site_id	SWS_FT_FI18
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:33408407, ECO:0000269|PubMed:34417297, ECO:0007744|PDB:6WTI, ECO:0007744|PDB:7CUB, ECO:0007744|PDB:7CUW, ECO:0007744|PDB:7N9Z

Chain	Residue	Details
E	ARG71
A	ARG71

---

site_id	SWS_FT_FI19
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:34417297, ECO:0007744|PDB:7CUW

Chain	Residue	Details
A	ASP75
E	ASP75

---

site_id	SWS_FT_FI20
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:34417297, ECO:0007744|PDB:7CUB, ECO:0007744|PDB:7CUW, ECO:0007744|PDB:7N9Z

Chain	Residue	Details
A	HIS98
E	HIS98

---

site_id	SWS_FT_FI21
Number of Residues	6
Details	BINDING: axial binding residue => ECO:0000269|PubMed:11017202, ECO:0000269|PubMed:33408407, ECO:0007744|PDB:1FFT, ECO:0007744|PDB:6WTI

Chain	Residue	Details
E	HIS419
E	HIS421
A	HIS106
A	HIS419
A	HIS421
E	HIS106

---

site_id	SWS_FT_FI22
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:11017202, ECO:0007744|PDB:1FFT

Chain	Residue	Details
A	TRP170
E	TRP170

---

site_id	SWS_FT_FI23
Number of Residues	10
Details	BINDING: BINDING => ECO:0000269|PubMed:11017202, ECO:0000269|PubMed:33408407, ECO:0007744|PDB:1FFT, ECO:0007744|PDB:6WTI

Chain	Residue	Details
A	HIS284
A	HIS333
A	HIS334
A	HIS411
A	ARG482
E	HIS284
E	HIS333
E	HIS334
E	HIS411
E	ARG482

---

site_id	SWS_FT_FI24
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:33408407, ECO:0007744|PDB:6WTI

Chain	Residue	Details
A	TYR288
A	ARG481
E	TYR288
E	ARG481

---

site_id	SWS_FT_FI25
Number of Residues	4
Details	CROSSLNK: 1'-histidyl-3'-tyrosine (His-Tyr) => ECO:0000269|PubMed:34417297

Chain	Residue	Details
A	HIS284
A	TYR288
E	HIS284
E	TYR288

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	23
Details	M-CSA 714

Chain	Residue	Details
A	TYR61	proton shuttle (general acid/base)
A	ARG71	electrostatic stabiliser
A	ASP75	electrostatic stabiliser
A	MET79	electron shuttle
A	PHE103	electron shuttle
A	ASN124	proton shuttle (general acid/base)
A	ASP135	proton shuttle (general acid/base)
A	ASN142	proton shuttle (general acid/base)
A	SER145	proton shuttle (general acid/base)
A	THR149	proton shuttle (general acid/base)
A	THR201	proton shuttle (general acid/base)
A	THR204	proton shuttle (general acid/base)
A	THR211	proton shuttle (general acid/base)
A	HIS284	metal ligand, modifies pKa
A	GLU286	proton shuttle (general acid/base)
A	TYR288	electron shuttle, proton shuttle (general acid/base)
A	SER299	proton shuttle (general acid/base)
A	SER315	proton shuttle (general acid/base)
A	THR359	proton shuttle (general acid/base)
A	LYS362	proton shuttle (general acid/base)
A	HIS419	electron shuttle
A	PHE420	electron shuttle
A	HIS421	electron shuttle

---

site_id	MCSA2
Number of Residues	23
Details	M-CSA 714

Chain	Residue	Details
E	TYR61	proton shuttle (general acid/base)
E	ARG71	electrostatic stabiliser
E	ASP75	electrostatic stabiliser
E	MET79	electron shuttle
E	PHE103	electron shuttle
E	ASN124	proton shuttle (general acid/base)
E	ASP135	proton shuttle (general acid/base)
E	ASN142	proton shuttle (general acid/base)
E	SER145	proton shuttle (general acid/base)
E	THR149	proton shuttle (general acid/base)
E	THR201	proton shuttle (general acid/base)
E	THR204	proton shuttle (general acid/base)
E	THR211	proton shuttle (general acid/base)
E	HIS284	metal ligand, modifies pKa
E	GLU286	proton shuttle (general acid/base)
E	TYR288	electron shuttle, proton shuttle (general acid/base)
E	SER299	proton shuttle (general acid/base)
E	SER315	proton shuttle (general acid/base)
E	THR359	proton shuttle (general acid/base)
E	LYS362	proton shuttle (general acid/base)
E	HIS419	electron shuttle
E	PHE420	electron shuttle
E	HIS421	electron shuttle

---