7LXV

Structure of human 20S proteasome with bound MPI-5

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000502	cellular_component	proteasome complex
A	0000932	cellular_component	P-body
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005839	cellular_component	proteasome core complex
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0009615	biological_process	response to virus
A	0019773	cellular_component	proteasome core complex, alpha-subunit complex
A	0034774	cellular_component	secretory granule lumen
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0051603	biological_process	proteolysis involved in protein catabolic process
A	0070062	cellular_component	extracellular exosome
A	1904813	cellular_component	ficolin-1-rich granule lumen
B	0000502	cellular_component	proteasome complex
B	0000932	cellular_component	P-body
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005839	cellular_component	proteasome core complex
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0019773	cellular_component	proteasome core complex, alpha-subunit complex
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0043231	cellular_component	intracellular membrane-bounded organelle
B	0051603	biological_process	proteolysis involved in protein catabolic process
B	0070062	cellular_component	extracellular exosome
C	0000502	cellular_component	proteasome complex
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005839	cellular_component	proteasome core complex
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0019773	cellular_component	proteasome core complex, alpha-subunit complex
C	0042802	molecular_function	identical protein binding
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0051603	biological_process	proteolysis involved in protein catabolic process
C	0070062	cellular_component	extracellular exosome
C	0098794	cellular_component	postsynapse
D	0000502	cellular_component	proteasome complex
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005839	cellular_component	proteasome core complex
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0019773	cellular_component	proteasome core complex, alpha-subunit complex
D	0034774	cellular_component	secretory granule lumen
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
D	0070062	cellular_component	extracellular exosome
D	1904813	cellular_component	ficolin-1-rich granule lumen
E	0000502	cellular_component	proteasome complex
E	0001530	molecular_function	lipopolysaccharide binding
E	0002376	biological_process	immune system process
E	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005813	cellular_component	centrosome
E	0005829	cellular_component	cytosol
E	0005839	cellular_component	proteasome core complex
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0019773	cellular_component	proteasome core complex, alpha-subunit complex
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0051603	biological_process	proteolysis involved in protein catabolic process
E	0070062	cellular_component	extracellular exosome
F	0000502	cellular_component	proteasome complex
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005839	cellular_component	proteasome core complex
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0019773	cellular_component	proteasome core complex, alpha-subunit complex
F	0031625	molecular_function	ubiquitin protein ligase binding
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0045202	cellular_component	synapse
F	0051603	biological_process	proteolysis involved in protein catabolic process
F	0052548	biological_process	regulation of endopeptidase activity
F	0070062	cellular_component	extracellular exosome
G	0000502	cellular_component	proteasome complex
G	0000932	cellular_component	P-body
G	0003723	molecular_function	RNA binding
G	0004175	molecular_function	endopeptidase activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005839	cellular_component	proteasome core complex
G	0005840	cellular_component	ribosome
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0016363	cellular_component	nuclear matrix
G	0019773	cellular_component	proteasome core complex, alpha-subunit complex
G	0030016	cellular_component	myofibril
G	0030017	cellular_component	sarcomere
G	0035639	molecular_function	purine ribonucleoside triphosphate binding
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0050727	biological_process	regulation of inflammatory response
G	0051059	molecular_function	NF-kappaB binding
G	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
G	0051603	biological_process	proteolysis involved in protein catabolic process
G	0070062	cellular_component	extracellular exosome
H	0004298	molecular_function	threonine-type endopeptidase activity
H	0005839	cellular_component	proteasome core complex
H	0051603	biological_process	proteolysis involved in protein catabolic process
I	0000502	cellular_component	proteasome complex
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005839	cellular_component	proteasome core complex
I	0019774	cellular_component	proteasome core complex, beta-subunit complex
I	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
I	0051603	biological_process	proteolysis involved in protein catabolic process
I	0070062	cellular_component	extracellular exosome
J	0000502	cellular_component	proteasome complex
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005839	cellular_component	proteasome core complex
J	0010243	biological_process	response to organonitrogen compound
J	0010498	biological_process	proteasomal protein catabolic process
J	0014070	biological_process	response to organic cyclic compound
J	0016020	cellular_component	membrane
J	0019774	cellular_component	proteasome core complex, beta-subunit complex
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0051603	biological_process	proteolysis involved in protein catabolic process
J	0070062	cellular_component	extracellular exosome
K	0004298	molecular_function	threonine-type endopeptidase activity
K	0005839	cellular_component	proteasome core complex
K	0051603	biological_process	proteolysis involved in protein catabolic process
L	0005839	cellular_component	proteasome core complex
L	0051603	biological_process	proteolysis involved in protein catabolic process
M	0005839	cellular_component	proteasome core complex
M	0051603	biological_process	proteolysis involved in protein catabolic process
N	0004298	molecular_function	threonine-type endopeptidase activity
N	0005839	cellular_component	proteasome core complex
N	0051603	biological_process	proteolysis involved in protein catabolic process
O	0000502	cellular_component	proteasome complex
O	0000932	cellular_component	P-body
O	0005515	molecular_function	protein binding
O	0005576	cellular_component	extracellular region
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005839	cellular_component	proteasome core complex
O	0006511	biological_process	ubiquitin-dependent protein catabolic process
O	0009615	biological_process	response to virus
O	0019773	cellular_component	proteasome core complex, alpha-subunit complex
O	0034774	cellular_component	secretory granule lumen
O	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
O	0051603	biological_process	proteolysis involved in protein catabolic process
O	0070062	cellular_component	extracellular exosome
O	1904813	cellular_component	ficolin-1-rich granule lumen
P	0000502	cellular_component	proteasome complex
P	0000932	cellular_component	P-body
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0005839	cellular_component	proteasome core complex
P	0006511	biological_process	ubiquitin-dependent protein catabolic process
P	0019773	cellular_component	proteasome core complex, alpha-subunit complex
P	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
P	0043231	cellular_component	intracellular membrane-bounded organelle
P	0051603	biological_process	proteolysis involved in protein catabolic process
P	0070062	cellular_component	extracellular exosome
Q	0000502	cellular_component	proteasome complex
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005839	cellular_component	proteasome core complex
Q	0006511	biological_process	ubiquitin-dependent protein catabolic process
Q	0019773	cellular_component	proteasome core complex, alpha-subunit complex
Q	0042802	molecular_function	identical protein binding
Q	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Q	0051603	biological_process	proteolysis involved in protein catabolic process
Q	0070062	cellular_component	extracellular exosome
Q	0098794	cellular_component	postsynapse
R	0000502	cellular_component	proteasome complex
R	0005515	molecular_function	protein binding
R	0005576	cellular_component	extracellular region
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0005839	cellular_component	proteasome core complex
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0019773	cellular_component	proteasome core complex, alpha-subunit complex
R	0034774	cellular_component	secretory granule lumen
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0051603	biological_process	proteolysis involved in protein catabolic process
R	0070062	cellular_component	extracellular exosome
R	1904813	cellular_component	ficolin-1-rich granule lumen
S	0000502	cellular_component	proteasome complex
S	0001530	molecular_function	lipopolysaccharide binding
S	0002376	biological_process	immune system process
S	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005737	cellular_component	cytoplasm
S	0005813	cellular_component	centrosome
S	0005829	cellular_component	cytosol
S	0005839	cellular_component	proteasome core complex
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0019773	cellular_component	proteasome core complex, alpha-subunit complex
S	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0051603	biological_process	proteolysis involved in protein catabolic process
S	0070062	cellular_component	extracellular exosome
T	0000502	cellular_component	proteasome complex
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0005839	cellular_component	proteasome core complex
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0019773	cellular_component	proteasome core complex, alpha-subunit complex
T	0031625	molecular_function	ubiquitin protein ligase binding
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0045202	cellular_component	synapse
T	0051603	biological_process	proteolysis involved in protein catabolic process
T	0052548	biological_process	regulation of endopeptidase activity
T	0070062	cellular_component	extracellular exosome
U	0000502	cellular_component	proteasome complex
U	0000932	cellular_component	P-body
U	0003723	molecular_function	RNA binding
U	0004175	molecular_function	endopeptidase activity
U	0005515	molecular_function	protein binding
U	0005634	cellular_component	nucleus
U	0005654	cellular_component	nucleoplasm
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0005839	cellular_component	proteasome core complex
U	0005840	cellular_component	ribosome
U	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0016363	cellular_component	nuclear matrix
U	0019773	cellular_component	proteasome core complex, alpha-subunit complex
U	0030016	cellular_component	myofibril
U	0030017	cellular_component	sarcomere
U	0035639	molecular_function	purine ribonucleoside triphosphate binding
U	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
U	0050727	biological_process	regulation of inflammatory response
U	0051059	molecular_function	NF-kappaB binding
U	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
U	0051603	biological_process	proteolysis involved in protein catabolic process
U	0070062	cellular_component	extracellular exosome
V	0004298	molecular_function	threonine-type endopeptidase activity
V	0005839	cellular_component	proteasome core complex
V	0051603	biological_process	proteolysis involved in protein catabolic process
W	0000502	cellular_component	proteasome complex
W	0005515	molecular_function	protein binding
W	0005634	cellular_component	nucleus
W	0005654	cellular_component	nucleoplasm
W	0005737	cellular_component	cytoplasm
W	0005829	cellular_component	cytosol
W	0005839	cellular_component	proteasome core complex
W	0019774	cellular_component	proteasome core complex, beta-subunit complex
W	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
W	0051603	biological_process	proteolysis involved in protein catabolic process
W	0070062	cellular_component	extracellular exosome
X	0000502	cellular_component	proteasome complex
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0005654	cellular_component	nucleoplasm
X	0005737	cellular_component	cytoplasm
X	0005829	cellular_component	cytosol
X	0005839	cellular_component	proteasome core complex
X	0010243	biological_process	response to organonitrogen compound
X	0010498	biological_process	proteasomal protein catabolic process
X	0014070	biological_process	response to organic cyclic compound
X	0016020	cellular_component	membrane
X	0019774	cellular_component	proteasome core complex, beta-subunit complex
X	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
X	0051603	biological_process	proteolysis involved in protein catabolic process
X	0070062	cellular_component	extracellular exosome
Y	0004298	molecular_function	threonine-type endopeptidase activity
Y	0005839	cellular_component	proteasome core complex
Y	0051603	biological_process	proteolysis involved in protein catabolic process
Z	0005839	cellular_component	proteasome core complex
Z	0051603	biological_process	proteolysis involved in protein catabolic process
a	0005839	cellular_component	proteasome core complex
a	0051603	biological_process	proteolysis involved in protein catabolic process
b	0004298	molecular_function	threonine-type endopeptidase activity
b	0005839	cellular_component	proteasome core complex
b	0051603	biological_process	proteolysis involved in protein catabolic process

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdlsaStFSPdGRvfQVEYAmkA

Chain	Residue	Details
F	TYR7-ALA29
E	TYR6-ALA28
A	TYR5-ALA27
G	PHE9-ALA31
D	TYR8-ALA30
C	TYR3-ALA25
B	TYR5-ALA27

---

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. LGVkFegGVVIAADmlgsygslarfrnis.RimrvnnstmlgasGdyAD

Chain	Residue	Details
a	LEU12-ASP59
b	MET4-ASP51
K	LEU4-ASP51
I	MET11-ASP58
H	ALA4-ASP51
B	LEU35-ASP82
J	ILE5-ASP52
L	LEU13-ASP60

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:25599644, ECO:0000269|PubMed:27493187

Chain	Residue	Details
N	THR1
b	THR1
L	SER134
Z	SER34
Z	SER40
Z	SER134

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
N	THR35
b	THR35
U	LYS102
U	LYS104
T	LYS205
T	LYS229

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
L	LYS176
Z	LYS176
P	LYS127
P	LYS176
O	SER13
O	SER15

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	CARBOHYD: O-linked (GlcNAc) serine => ECO:0000250

Chain	Residue	Details
L	SER30
L	SER181
Z	SER30
Z	SER181

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Chain	Residue	Details
E	LYS115
R	SER63
E	LYS208
S	LYS115
S	LYS208

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	CARBOHYD: O-linked (GlcNAc) serine => ECO:0000250

Chain	Residue	Details
D	SER198
R	SER198

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P17220

Chain	Residue	Details
A	TYR120
O	TYR120

---