7BC7
Cryo-EM structure of the proton coupled folate transporter at pH 6.0 bound to pemetrexed
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005768 | cellular_component | endosome |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0010008 | cellular_component | endosome membrane |
| A | 0015293 | molecular_function | symporter activity |
| A | 0015350 | molecular_function | methotrexate transmembrane transporter activity |
| A | 0016323 | cellular_component | basolateral plasma membrane |
| A | 0016324 | cellular_component | apical plasma membrane |
| A | 0022857 | molecular_function | transmembrane transporter activity |
| A | 0051958 | biological_process | methotrexate transport |
| A | 0055085 | biological_process | transmembrane transport |
| A | 0140211 | molecular_function | folic acid:proton symporter activity |
| A | 1904447 | biological_process | folate import across plasma membrane |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 130 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | MET1-VAL29 | |
| A | ASP117-GLY120 | |
| A | GLY163-ARG185 | |
| A | LEU236-LYS274 | |
| A | GLN334-ASP339 | |
| A | ALA385-GLN396 | |
| A | TRP450-GLY473 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 18 |
| Details | TRANSMEM: Helical; Name=TM1 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | GLU30-THR48 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 71 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | GLN49-VAL90 | |
| A | TYR144-HIS148 | |
| A | LYS212-TYR216 | |
| A | LEU298-ASP310 | |
| A | ALA360-THR363 | |
| A | PRO423-ARG430 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 25 |
| Details | TRANSMEM: Helical; Name=TM2 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | ALA91-SER116 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 22 |
| Details | TRANSMEM: Helical; Name=TM3 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | ARG121-MET143 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 13 |
| Details | TRANSMEM: Helical; Name=TM4 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | VAL149-LEU162 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 25 |
| Details | TRANSMEM: Helical; Name=TM5 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | THR186-ARG211 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 18 |
| Details | TRANSMEM: Helical; Name=TM6 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | ILE217-ALA235 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 22 |
| Details | TRANSMEM: Helical; Name=TM7 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | LEU275-VAL297 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 22 |
| Details | TRANSMEM: Helical; Name=TM8 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | LEU311-LEU333 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 19 |
| Details | TRANSMEM: Helical; Name=TM9 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | THR340-LEU359 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 20 |
| Details | TRANSMEM: Helical; Name=TM10 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | PRO364-ARG384 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 25 |
| Details | TRANSMEM: Helical; Name=TM11 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | GLY397-TYR422 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 18 |
| Details | TRANSMEM: Helical; Name=TM12 => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC6, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | GLY431-GLY449 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:34040256, ECO:0007744|PDB:7BC7 |
| Chain | Residue | Details |
| A | ASN98 | |
| A | GLU193 | |
| A | TYR323 | |
| A | GLU407 | |
| A | SER411 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 1 |
| Details | BINDING: reversibly protonated residue during proton transport => ECO:0000269|PubMed:34040256 |
| Chain | Residue | Details |
| A | ASP164 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 1 |
| Details | BINDING: reversibly protonated residue during proton transport => ECO:0000250|UniProtKB:Q96NT5 |
| Chain | Residue | Details |
| A | HIS289 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255|PROSITE-ProRule:PRU00498 |
| Chain | Residue | Details |
| A | ASN65 | |
| A | ASN74 |
