7A6H

Cryo-EM structure of human apo RNA Polymerase III

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0001056	molecular_function	RNA polymerase III activity
A	0003677	molecular_function	DNA binding
A	0003682	molecular_function	chromatin binding
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005666	cellular_component	RNA polymerase III complex
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006351	biological_process	DNA-templated transcription
A	0008270	molecular_function	zinc ion binding
A	0016020	cellular_component	membrane
A	0016779	molecular_function	nucleotidyltransferase activity
A	0031981	cellular_component	nuclear lumen
A	0032728	biological_process	positive regulation of interferon-beta production
A	0032991	cellular_component	protein-containing complex
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0042797	biological_process	tRNA transcription by RNA polymerase III
A	0045087	biological_process	innate immune response
A	0046872	molecular_function	metal ion binding
A	0051607	biological_process	defense response to virus
A	0071667	molecular_function	DNA/RNA hybrid binding
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0001056	molecular_function	RNA polymerase III activity
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005666	cellular_component	RNA polymerase III complex
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006351	biological_process	DNA-templated transcription
B	0008270	molecular_function	zinc ion binding
B	0016779	molecular_function	nucleotidyltransferase activity
B	0032549	molecular_function	ribonucleoside binding
B	0032728	biological_process	positive regulation of interferon-beta production
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0042796	biological_process	snRNA transcription by RNA polymerase III
B	0045087	biological_process	innate immune response
B	0045089	biological_process	positive regulation of innate immune response
B	0046872	molecular_function	metal ion binding
B	0051607	biological_process	defense response to virus
B	0071667	molecular_function	DNA/RNA hybrid binding
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0001054	molecular_function	RNA polymerase I activity
C	0001056	molecular_function	RNA polymerase III activity
C	0001650	cellular_component	fibrillar center
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005666	cellular_component	RNA polymerase III complex
C	0005730	cellular_component	nucleolus
C	0005736	cellular_component	RNA polymerase I complex
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006351	biological_process	DNA-templated transcription
C	0006360	biological_process	transcription by RNA polymerase I
C	0006383	biological_process	transcription by RNA polymerase III
C	0046983	molecular_function	protein dimerization activity
D	0000166	molecular_function	nucleotide binding
D	0000428	cellular_component	DNA-directed RNA polymerase complex
D	0001635	molecular_function	calcitonin gene-related peptide receptor activity
D	0001669	cellular_component	acrosomal vesicle
D	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005666	cellular_component	RNA polymerase III complex
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006352	biological_process	DNA-templated transcription initiation
D	0006383	biological_process	transcription by RNA polymerase III
D	0006384	biological_process	transcription initiation at RNA polymerase III promoter
D	0007218	biological_process	neuropeptide signaling pathway
D	0009360	cellular_component	DNA polymerase III complex
D	0030880	cellular_component	RNA polymerase complex
D	0044237	biological_process	cellular metabolic process
D	0045087	biological_process	innate immune response
D	0051607	biological_process	defense response to virus
E	0000428	cellular_component	DNA-directed RNA polymerase complex
E	0001054	molecular_function	RNA polymerase I activity
E	0001055	molecular_function	RNA polymerase II activity
E	0001056	molecular_function	RNA polymerase III activity
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005665	cellular_component	RNA polymerase II, core complex
E	0005666	cellular_component	RNA polymerase III complex
E	0005730	cellular_component	nucleolus
E	0005736	cellular_component	RNA polymerase I complex
E	0005829	cellular_component	cytosol
E	0006351	biological_process	DNA-templated transcription
E	0006362	biological_process	transcription elongation by RNA polymerase I
E	0006366	biological_process	transcription by RNA polymerase II
E	0016591	cellular_component	RNA polymerase II, holoenzyme
E	0042797	biological_process	tRNA transcription by RNA polymerase III
E	0050821	biological_process	protein stabilization
E	1990062	cellular_component	RPAP3/R2TP/prefoldin-like complex
F	0000428	cellular_component	DNA-directed RNA polymerase complex
F	0001054	molecular_function	RNA polymerase I activity
F	0001055	molecular_function	RNA polymerase II activity
F	0001056	molecular_function	RNA polymerase III activity
F	0001650	cellular_component	fibrillar center
F	0003677	molecular_function	DNA binding
F	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005665	cellular_component	RNA polymerase II, core complex
F	0005666	cellular_component	RNA polymerase III complex
F	0005730	cellular_component	nucleolus
F	0005736	cellular_component	RNA polymerase I complex
F	0005829	cellular_component	cytosol
F	0006351	biological_process	DNA-templated transcription
F	0006360	biological_process	transcription by RNA polymerase I
F	0006366	biological_process	transcription by RNA polymerase II
F	0042797	biological_process	tRNA transcription by RNA polymerase III
G	0000428	cellular_component	DNA-directed RNA polymerase complex
G	0003677	molecular_function	DNA binding
G	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005666	cellular_component	RNA polymerase III complex
G	0005813	cellular_component	centrosome
G	0005829	cellular_component	cytosol
G	0006139	biological_process	nucleobase-containing compound metabolic process
G	0006351	biological_process	DNA-templated transcription
G	0006352	biological_process	DNA-templated transcription initiation
G	0006383	biological_process	transcription by RNA polymerase III
G	0006384	biological_process	transcription initiation at RNA polymerase III promoter
G	0043231	cellular_component	intracellular membrane-bounded organelle
G	0045087	biological_process	innate immune response
G	0051607	biological_process	defense response to virus
H	0000428	cellular_component	DNA-directed RNA polymerase complex
H	0001054	molecular_function	RNA polymerase I activity
H	0001055	molecular_function	RNA polymerase II activity
H	0001056	molecular_function	RNA polymerase III activity
H	0003677	molecular_function	DNA binding
H	0003697	molecular_function	single-stranded DNA binding
H	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005665	cellular_component	RNA polymerase II, core complex
H	0005666	cellular_component	RNA polymerase III complex
H	0005730	cellular_component	nucleolus
H	0005736	cellular_component	RNA polymerase I complex
H	0005829	cellular_component	cytosol
H	0006351	biological_process	DNA-templated transcription
H	0006360	biological_process	transcription by RNA polymerase I
H	0006366	biological_process	transcription by RNA polymerase II
H	0006383	biological_process	transcription by RNA polymerase III
H	0032993	cellular_component	protein-DNA complex
I	0000428	cellular_component	DNA-directed RNA polymerase complex
I	0001056	molecular_function	RNA polymerase III activity
I	0003676	molecular_function	nucleic acid binding
I	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005666	cellular_component	RNA polymerase III complex
I	0005829	cellular_component	cytosol
I	0006351	biological_process	DNA-templated transcription
I	0006383	biological_process	transcription by RNA polymerase III
I	0006386	biological_process	termination of RNA polymerase III transcription
I	0008270	molecular_function	zinc ion binding
I	0045087	biological_process	innate immune response
I	0046872	molecular_function	metal ion binding
I	0051607	biological_process	defense response to virus
I	0055029	cellular_component	nuclear DNA-directed RNA polymerase complex
J	0000428	cellular_component	DNA-directed RNA polymerase complex
J	0001054	molecular_function	RNA polymerase I activity
J	0001055	molecular_function	RNA polymerase II activity
J	0001056	molecular_function	RNA polymerase III activity
J	0003677	molecular_function	DNA binding
J	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005665	cellular_component	RNA polymerase II, core complex
J	0005666	cellular_component	RNA polymerase III complex
J	0005730	cellular_component	nucleolus
J	0005736	cellular_component	RNA polymerase I complex
J	0005829	cellular_component	cytosol
J	0006351	biological_process	DNA-templated transcription
J	0006356	biological_process	regulation of transcription by RNA polymerase I
J	0006360	biological_process	transcription by RNA polymerase I
J	0006366	biological_process	transcription by RNA polymerase II
J	0008270	molecular_function	zinc ion binding
J	0042797	biological_process	tRNA transcription by RNA polymerase III
J	0046872	molecular_function	metal ion binding
K	0000428	cellular_component	DNA-directed RNA polymerase complex
K	0001054	molecular_function	RNA polymerase I activity
K	0001056	molecular_function	RNA polymerase III activity
K	0003677	molecular_function	DNA binding
K	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005666	cellular_component	RNA polymerase III complex
K	0005730	cellular_component	nucleolus
K	0005736	cellular_component	RNA polymerase I complex
K	0006351	biological_process	DNA-templated transcription
K	0006362	biological_process	transcription elongation by RNA polymerase I
K	0006383	biological_process	transcription by RNA polymerase III
K	0046983	molecular_function	protein dimerization activity
K	0055029	cellular_component	nuclear DNA-directed RNA polymerase complex
L	0000428	cellular_component	DNA-directed RNA polymerase complex
L	0001054	molecular_function	RNA polymerase I activity
L	0001055	molecular_function	RNA polymerase II activity
L	0001056	molecular_function	RNA polymerase III activity
L	0003677	molecular_function	DNA binding
L	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005665	cellular_component	RNA polymerase II, core complex
L	0005666	cellular_component	RNA polymerase III complex
L	0005730	cellular_component	nucleolus
L	0005736	cellular_component	RNA polymerase I complex
L	0005829	cellular_component	cytosol
L	0006351	biological_process	DNA-templated transcription
L	0006356	biological_process	regulation of transcription by RNA polymerase I
L	0006360	biological_process	transcription by RNA polymerase I
L	0006366	biological_process	transcription by RNA polymerase II
L	0006383	biological_process	transcription by RNA polymerase III
L	0008270	molecular_function	zinc ion binding
L	0046872	molecular_function	metal ion binding
M	0000428	cellular_component	DNA-directed RNA polymerase complex
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005666	cellular_component	RNA polymerase III complex
M	0005829	cellular_component	cytosol
M	0006351	biological_process	DNA-templated transcription
M	0042797	biological_process	tRNA transcription by RNA polymerase III
M	0045087	biological_process	innate immune response
M	0051607	biological_process	defense response to virus
N	0000428	cellular_component	DNA-directed RNA polymerase complex
N	0003677	molecular_function	DNA binding
N	0003682	molecular_function	chromatin binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005666	cellular_component	RNA polymerase III complex
N	0005829	cellular_component	cytosol
N	0006383	biological_process	transcription by RNA polymerase III
N	0032728	biological_process	positive regulation of interferon-beta production
N	0042797	biological_process	tRNA transcription by RNA polymerase III
N	0045087	biological_process	innate immune response
N	0045089	biological_process	positive regulation of innate immune response
N	0051607	biological_process	defense response to virus
O	0000428	cellular_component	DNA-directed RNA polymerase complex
O	0003677	molecular_function	DNA binding
O	0003697	molecular_function	single-stranded DNA binding
O	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005666	cellular_component	RNA polymerase III complex
O	0005829	cellular_component	cytosol
O	0006351	biological_process	DNA-templated transcription
O	0006359	biological_process	regulation of transcription by RNA polymerase III
O	0032728	biological_process	positive regulation of interferon-beta production
O	0045087	biological_process	innate immune response
O	0045089	biological_process	positive regulation of innate immune response
O	0051607	biological_process	defense response to virus
P	0000428	cellular_component	DNA-directed RNA polymerase complex
P	0003677	molecular_function	DNA binding
P	0003690	molecular_function	double-stranded DNA binding
P	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005666	cellular_component	RNA polymerase III complex
P	0005829	cellular_component	cytosol
P	0006359	biological_process	regulation of transcription by RNA polymerase III
P	0006383	biological_process	transcription by RNA polymerase III
P	0032728	biological_process	positive regulation of interferon-beta production
P	0045087	biological_process	innate immune response
P	0045089	biological_process	positive regulation of innate immune response
P	0051539	molecular_function	4 iron, 4 sulfur cluster binding
P	0051607	biological_process	defense response to virus
Q	0000428	cellular_component	DNA-directed RNA polymerase complex
Q	0003682	molecular_function	chromatin binding
Q	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005666	cellular_component	RNA polymerase III complex
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0006359	biological_process	regulation of transcription by RNA polymerase III
Q	0006383	biological_process	transcription by RNA polymerase III
Q	0008283	biological_process	cell population proliferation
Q	0016604	cellular_component	nuclear body
Q	0032728	biological_process	positive regulation of interferon-beta production
Q	0045087	biological_process	innate immune response
Q	0045089	biological_process	positive regulation of innate immune response
Q	0051607	biological_process	defense response to virus

Functional Information from PROSITE/UniProt

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKFSSrHGQKGV

Chain	Residue	Details
B	GLY894-VAL906

---

site_id	PS01110
Number of Residues	14
Details	RNA_POL_H_23KD RNA polymerases H / 23 Kd subunits signature. HELVPEHvvMtkEE

Chain	Residue	Details
E	HIS142-GLU155

---

site_id	PS00446
Number of Residues	41
Details	RNA_POL_D_30KD RNA polymerases D / 30 to 40 Kd subunits signature. NAFRRillaevptmAvekVlvynNtSivqDEiLAhRLGLIP

Chain	Residue	Details
C	ASN74-PRO114

---

site_id	PS01154
Number of Residues	32
Details	RNA_POL_L_13KD RNA polymerases L / 13 to 16 Kd subunits signature. LheEdHTLgNsLrymImknpeVefcgYtttHP

Chain	Residue	Details
K	LEU44-PRO75

---

site_id	PS01111
Number of Residues	15
Details	RNA_POL_K_14KD RNA polymerases K / 14 to 18 Kd subunits signature. TkYErARvLGtRAlQ

Chain	Residue	Details
F	THR58-GLN72

---

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. FRCVVFH

Chain	Residue	Details
G	PHE72-HIS78

---

site_id	PS01112
Number of Residues	10
Details	RNA_POL_N_8KD RNA polymerases N / 8 Kd subunits signature. IIPVrCFTCG

Chain	Residue	Details
J	ILE2-GLY11

---

site_id	PS00466
Number of Residues	38
Details	ZF_TFIIS_1 Zinc finger TFIIS-type signature. CpkCehprayfmqLQTRSaDEPmttfykCcnaq.CghrW

Chain	Residue	Details
I	CYS69-TRP106

---

site_id	PS01030
Number of Residues	25
Details	RNA_POL_M_15KD RNA polymerases M / 15 Kd subunits signature. FCpGCGNGLiveegqrchrfs..CntC

Chain	Residue	Details
I	PHE4-CYS28

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
J	CYS10
J	CYS44
Q	THR133

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692

Chain	Residue	Details
B	ARG1046
B	ASP432
Q	SER157
B	ARG213
B	LYS1019

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:33558764, ECO:0000269|PubMed:33558766, ECO:0007744|PDB:7D58

Chain	Residue	Details
P	CYS307
P	CYS296
N	ARG99

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0007744|PubMed:22814378

Chain	Residue	Details
N	LYS152
N	LYS160
N	LYS190
N	LYS199
N	LYS310
P	ALA2

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS1323
P	LYS5
N	LYS396

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
P	LYS7
N	LYS78

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0000269|PubMed:33558764, ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
N	LYS141
M	LYS432

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	ARG1305
M	LYS659
N	LYS206

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
N	LYS302
N	LYS220

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
N	LYS285
A	ASP503

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:33558764, ECO:0000269|PubMed:33674783, ECO:0000269|PubMed:34675218, ECO:0007744|PDB:7AE1, ECO:0007744|PDB:7DN3, ECO:0007744|PDB:7FJJ

Chain	Residue	Details
B	CYS1083

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:33558764, ECO:0000269|PubMed:33558766, ECO:0000269|PubMed:33674783, ECO:0000269|PubMed:34675218, ECO:0007744|PDB:7AE1, ECO:0007744|PDB:7D58, ECO:0007744|PDB:7DN3, ECO:0007744|PDB:7FJJ

Chain	Residue	Details
B	CYS1095
B	CYS1092

---

site_id	SWS_FT_FI13
Number of Residues	1
Details	SITE: Critical in trapping poly(dT) in Pol III-mediated transcription termination => ECO:0000305|PubMed:34675218

Chain	Residue	Details
B	PHE429

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS445

---