6WLZ

The V1 region of human V-ATPase in state 1 (focused refinement)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000139	cellular_component	Golgi membrane
A	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005764	cellular_component	lysosome
A	0005765	cellular_component	lysosomal membrane
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005902	cellular_component	microvillus
A	0006879	biological_process	intracellular iron ion homeostasis
A	0007035	biological_process	vacuolar acidification
A	0007042	biological_process	lysosomal lumen acidification
A	0010008	cellular_component	endosome membrane
A	0015986	biological_process	proton motive force-driven ATP synthesis
A	0016020	cellular_component	membrane
A	0016241	biological_process	regulation of macroautophagy
A	0016324	cellular_component	apical plasma membrane
A	0016469	cellular_component	proton-transporting two-sector ATPase complex
A	0016887	molecular_function	ATP hydrolysis activity
A	0030133	cellular_component	transport vesicle
A	0030141	cellular_component	secretory granule
A	0030665	cellular_component	clathrin-coated vesicle membrane
A	0031090	cellular_component	organelle membrane
A	0031410	cellular_component	cytoplasmic vesicle
A	0033176	cellular_component	proton-transporting V-type ATPase complex
A	0033180	cellular_component	proton-transporting V-type ATPase, V1 domain
A	0036295	biological_process	cellular response to increased oxygen levels
A	0043231	cellular_component	intracellular membrane-bounded organelle
A	0046034	biological_process	ATP metabolic process
A	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
A	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
A	0048388	biological_process	endosomal lumen acidification
A	0051452	biological_process	intracellular pH reduction
A	0061795	biological_process	Golgi lumen acidification
A	0070062	cellular_component	extracellular exosome
A	0097401	biological_process	synaptic vesicle lumen acidification
A	0098850	cellular_component	extrinsic component of synaptic vesicle membrane
A	1902495	cellular_component	transmembrane transporter complex
A	1902600	biological_process	proton transmembrane transport
A	1904949	cellular_component	ATPase complex
B	0000139	cellular_component	Golgi membrane
B	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005764	cellular_component	lysosome
B	0005765	cellular_component	lysosomal membrane
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0005902	cellular_component	microvillus
B	0006879	biological_process	intracellular iron ion homeostasis
B	0007035	biological_process	vacuolar acidification
B	0007042	biological_process	lysosomal lumen acidification
B	0010008	cellular_component	endosome membrane
B	0015986	biological_process	proton motive force-driven ATP synthesis
B	0016020	cellular_component	membrane
B	0016241	biological_process	regulation of macroautophagy
B	0016324	cellular_component	apical plasma membrane
B	0016469	cellular_component	proton-transporting two-sector ATPase complex
B	0016887	molecular_function	ATP hydrolysis activity
B	0030133	cellular_component	transport vesicle
B	0030141	cellular_component	secretory granule
B	0030665	cellular_component	clathrin-coated vesicle membrane
B	0031090	cellular_component	organelle membrane
B	0031410	cellular_component	cytoplasmic vesicle
B	0033176	cellular_component	proton-transporting V-type ATPase complex
B	0033180	cellular_component	proton-transporting V-type ATPase, V1 domain
B	0036295	biological_process	cellular response to increased oxygen levels
B	0043231	cellular_component	intracellular membrane-bounded organelle
B	0046034	biological_process	ATP metabolic process
B	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
B	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
B	0048388	biological_process	endosomal lumen acidification
B	0051452	biological_process	intracellular pH reduction
B	0061795	biological_process	Golgi lumen acidification
B	0070062	cellular_component	extracellular exosome
B	0097401	biological_process	synaptic vesicle lumen acidification
B	0098850	cellular_component	extrinsic component of synaptic vesicle membrane
B	1902495	cellular_component	transmembrane transporter complex
B	1902600	biological_process	proton transmembrane transport
B	1904949	cellular_component	ATPase complex
C	0000139	cellular_component	Golgi membrane
C	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005764	cellular_component	lysosome
C	0005765	cellular_component	lysosomal membrane
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005902	cellular_component	microvillus
C	0006879	biological_process	intracellular iron ion homeostasis
C	0007035	biological_process	vacuolar acidification
C	0007042	biological_process	lysosomal lumen acidification
C	0010008	cellular_component	endosome membrane
C	0015986	biological_process	proton motive force-driven ATP synthesis
C	0016020	cellular_component	membrane
C	0016241	biological_process	regulation of macroautophagy
C	0016324	cellular_component	apical plasma membrane
C	0016469	cellular_component	proton-transporting two-sector ATPase complex
C	0016887	molecular_function	ATP hydrolysis activity
C	0030133	cellular_component	transport vesicle
C	0030141	cellular_component	secretory granule
C	0030665	cellular_component	clathrin-coated vesicle membrane
C	0031090	cellular_component	organelle membrane
C	0031410	cellular_component	cytoplasmic vesicle
C	0033176	cellular_component	proton-transporting V-type ATPase complex
C	0033180	cellular_component	proton-transporting V-type ATPase, V1 domain
C	0036295	biological_process	cellular response to increased oxygen levels
C	0043231	cellular_component	intracellular membrane-bounded organelle
C	0046034	biological_process	ATP metabolic process
C	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
C	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
C	0048388	biological_process	endosomal lumen acidification
C	0051452	biological_process	intracellular pH reduction
C	0061795	biological_process	Golgi lumen acidification
C	0070062	cellular_component	extracellular exosome
C	0097401	biological_process	synaptic vesicle lumen acidification
C	0098850	cellular_component	extrinsic component of synaptic vesicle membrane
C	1902495	cellular_component	transmembrane transporter complex
C	1902600	biological_process	proton transmembrane transport
C	1904949	cellular_component	ATPase complex
D	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
D	0001726	cellular_component	ruffle
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005765	cellular_component	lysosomal membrane
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0005902	cellular_component	microvillus
D	0007035	biological_process	vacuolar acidification
D	0015078	molecular_function	proton transmembrane transporter activity
D	0016241	biological_process	regulation of macroautophagy
D	0016324	cellular_component	apical plasma membrane
D	0016469	cellular_component	proton-transporting two-sector ATPase complex
D	0030665	cellular_component	clathrin-coated vesicle membrane
D	0030672	cellular_component	synaptic vesicle membrane
D	0031090	cellular_component	organelle membrane
D	0031410	cellular_component	cytoplasmic vesicle
D	0033180	cellular_component	proton-transporting V-type ATPase, V1 domain
D	0042470	cellular_component	melanosome
D	0043231	cellular_component	intracellular membrane-bounded organelle
D	0045202	cellular_component	synapse
D	0046034	biological_process	ATP metabolic process
D	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
D	0070062	cellular_component	extracellular exosome
D	0097401	biological_process	synaptic vesicle lumen acidification
D	0098850	cellular_component	extrinsic component of synaptic vesicle membrane
D	1902600	biological_process	proton transmembrane transport
E	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
E	0001726	cellular_component	ruffle
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005765	cellular_component	lysosomal membrane
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0005902	cellular_component	microvillus
E	0007035	biological_process	vacuolar acidification
E	0015078	molecular_function	proton transmembrane transporter activity
E	0016241	biological_process	regulation of macroautophagy
E	0016324	cellular_component	apical plasma membrane
E	0016469	cellular_component	proton-transporting two-sector ATPase complex
E	0030665	cellular_component	clathrin-coated vesicle membrane
E	0030672	cellular_component	synaptic vesicle membrane
E	0031090	cellular_component	organelle membrane
E	0031410	cellular_component	cytoplasmic vesicle
E	0033180	cellular_component	proton-transporting V-type ATPase, V1 domain
E	0042470	cellular_component	melanosome
E	0043231	cellular_component	intracellular membrane-bounded organelle
E	0045202	cellular_component	synapse
E	0046034	biological_process	ATP metabolic process
E	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
E	0070062	cellular_component	extracellular exosome
E	0097401	biological_process	synaptic vesicle lumen acidification
E	0098850	cellular_component	extrinsic component of synaptic vesicle membrane
E	1902600	biological_process	proton transmembrane transport
F	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
F	0001726	cellular_component	ruffle
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005765	cellular_component	lysosomal membrane
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0005902	cellular_component	microvillus
F	0007035	biological_process	vacuolar acidification
F	0015078	molecular_function	proton transmembrane transporter activity
F	0016241	biological_process	regulation of macroautophagy
F	0016324	cellular_component	apical plasma membrane
F	0016469	cellular_component	proton-transporting two-sector ATPase complex
F	0030665	cellular_component	clathrin-coated vesicle membrane
F	0030672	cellular_component	synaptic vesicle membrane
F	0031090	cellular_component	organelle membrane
F	0031410	cellular_component	cytoplasmic vesicle
F	0033180	cellular_component	proton-transporting V-type ATPase, V1 domain
F	0042470	cellular_component	melanosome
F	0043231	cellular_component	intracellular membrane-bounded organelle
F	0045202	cellular_component	synapse
F	0046034	biological_process	ATP metabolic process
F	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
F	0070062	cellular_component	extracellular exosome
F	0097401	biological_process	synaptic vesicle lumen acidification
F	0098850	cellular_component	extrinsic component of synaptic vesicle membrane
F	1902600	biological_process	proton transmembrane transport
G	0000139	cellular_component	Golgi membrane
G	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
G	0005515	molecular_function	protein binding
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005765	cellular_component	lysosomal membrane
G	0005813	cellular_component	centrosome
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0005886	cellular_component	plasma membrane
G	0005929	cellular_component	cilium
G	0007035	biological_process	vacuolar acidification
G	0007042	biological_process	lysosomal lumen acidification
G	0010008	cellular_component	endosome membrane
G	0016020	cellular_component	membrane
G	0016241	biological_process	regulation of macroautophagy
G	0030030	biological_process	cell projection organization
G	0030665	cellular_component	clathrin-coated vesicle membrane
G	0031410	cellular_component	cytoplasmic vesicle
G	0033176	cellular_component	proton-transporting V-type ATPase complex
G	0035579	cellular_component	specific granule membrane
G	0042995	cellular_component	cell projection
G	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
G	0048388	biological_process	endosomal lumen acidification
G	0051452	biological_process	intracellular pH reduction
G	0060271	biological_process	cilium assembly
G	0061512	biological_process	protein localization to cilium
G	0061795	biological_process	Golgi lumen acidification
G	0070062	cellular_component	extracellular exosome
G	0097401	biological_process	synaptic vesicle lumen acidification
G	0098850	cellular_component	extrinsic component of synaptic vesicle membrane
G	1902495	cellular_component	transmembrane transporter complex
G	1902600	biological_process	proton transmembrane transport
H	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005765	cellular_component	lysosomal membrane
H	0005768	cellular_component	endosome
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0005902	cellular_component	microvillus
H	0016241	biological_process	regulation of macroautophagy
H	0016324	cellular_component	apical plasma membrane
H	0016469	cellular_component	proton-transporting two-sector ATPase complex
H	0030665	cellular_component	clathrin-coated vesicle membrane
H	0030672	cellular_component	synaptic vesicle membrane
H	0031410	cellular_component	cytoplasmic vesicle
H	0033178	cellular_component	proton-transporting two-sector ATPase complex, catalytic domain
H	0045202	cellular_component	synapse
H	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
H	0051117	molecular_function	ATPase binding
H	0070062	cellular_component	extracellular exosome
H	0097401	biological_process	synaptic vesicle lumen acidification
H	1902600	biological_process	proton transmembrane transport
I	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005765	cellular_component	lysosomal membrane
I	0005768	cellular_component	endosome
I	0005829	cellular_component	cytosol
I	0005886	cellular_component	plasma membrane
I	0005902	cellular_component	microvillus
I	0016241	biological_process	regulation of macroautophagy
I	0016324	cellular_component	apical plasma membrane
I	0016469	cellular_component	proton-transporting two-sector ATPase complex
I	0030665	cellular_component	clathrin-coated vesicle membrane
I	0030672	cellular_component	synaptic vesicle membrane
I	0031410	cellular_component	cytoplasmic vesicle
I	0033178	cellular_component	proton-transporting two-sector ATPase complex, catalytic domain
I	0045202	cellular_component	synapse
I	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
I	0051117	molecular_function	ATPase binding
I	0070062	cellular_component	extracellular exosome
I	0097401	biological_process	synaptic vesicle lumen acidification
I	1902600	biological_process	proton transmembrane transport
J	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005765	cellular_component	lysosomal membrane
J	0005768	cellular_component	endosome
J	0005829	cellular_component	cytosol
J	0005886	cellular_component	plasma membrane
J	0005902	cellular_component	microvillus
J	0016241	biological_process	regulation of macroautophagy
J	0016324	cellular_component	apical plasma membrane
J	0016469	cellular_component	proton-transporting two-sector ATPase complex
J	0030665	cellular_component	clathrin-coated vesicle membrane
J	0030672	cellular_component	synaptic vesicle membrane
J	0031410	cellular_component	cytoplasmic vesicle
J	0033178	cellular_component	proton-transporting two-sector ATPase complex, catalytic domain
J	0045202	cellular_component	synapse
J	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
J	0051117	molecular_function	ATPase binding
J	0070062	cellular_component	extracellular exosome
J	0097401	biological_process	synaptic vesicle lumen acidification
J	1902600	biological_process	proton transmembrane transport
K	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
K	0005515	molecular_function	protein binding
K	0005765	cellular_component	lysosomal membrane
K	0005829	cellular_component	cytosol
K	0005886	cellular_component	plasma membrane
K	0006879	biological_process	intracellular iron ion homeostasis
K	0016241	biological_process	regulation of macroautophagy
K	0016324	cellular_component	apical plasma membrane
K	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
K	0016887	molecular_function	ATP hydrolysis activity
K	0030672	cellular_component	synaptic vesicle membrane
K	0033176	cellular_component	proton-transporting V-type ATPase complex
K	0033180	cellular_component	proton-transporting V-type ATPase, V1 domain
K	0036295	biological_process	cellular response to increased oxygen levels
K	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
K	0051117	molecular_function	ATPase binding
K	0070062	cellular_component	extracellular exosome
K	0097401	biological_process	synaptic vesicle lumen acidification
K	1902495	cellular_component	transmembrane transporter complex
K	1902600	biological_process	proton transmembrane transport
K	1904949	cellular_component	ATPase complex
L	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
L	0005515	molecular_function	protein binding
L	0005765	cellular_component	lysosomal membrane
L	0005829	cellular_component	cytosol
L	0005886	cellular_component	plasma membrane
L	0006879	biological_process	intracellular iron ion homeostasis
L	0016241	biological_process	regulation of macroautophagy
L	0016324	cellular_component	apical plasma membrane
L	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
L	0016887	molecular_function	ATP hydrolysis activity
L	0030672	cellular_component	synaptic vesicle membrane
L	0033176	cellular_component	proton-transporting V-type ATPase complex
L	0033180	cellular_component	proton-transporting V-type ATPase, V1 domain
L	0036295	biological_process	cellular response to increased oxygen levels
L	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
L	0051117	molecular_function	ATPase binding
L	0070062	cellular_component	extracellular exosome
L	0097401	biological_process	synaptic vesicle lumen acidification
L	1902495	cellular_component	transmembrane transporter complex
L	1902600	biological_process	proton transmembrane transport
L	1904949	cellular_component	ATPase complex
M	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
M	0005515	molecular_function	protein binding
M	0005765	cellular_component	lysosomal membrane
M	0005829	cellular_component	cytosol
M	0005886	cellular_component	plasma membrane
M	0006879	biological_process	intracellular iron ion homeostasis
M	0016241	biological_process	regulation of macroautophagy
M	0016324	cellular_component	apical plasma membrane
M	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
M	0016887	molecular_function	ATP hydrolysis activity
M	0030672	cellular_component	synaptic vesicle membrane
M	0033176	cellular_component	proton-transporting V-type ATPase complex
M	0033180	cellular_component	proton-transporting V-type ATPase, V1 domain
M	0036295	biological_process	cellular response to increased oxygen levels
M	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
M	0051117	molecular_function	ATPase binding
M	0070062	cellular_component	extracellular exosome
M	0097401	biological_process	synaptic vesicle lumen acidification
M	1902495	cellular_component	transmembrane transporter complex
M	1902600	biological_process	proton transmembrane transport
M	1904949	cellular_component	ATPase complex
N	0000139	cellular_component	Golgi membrane
N	0000221	cellular_component	vacuolar proton-transporting V-type ATPase, V1 domain
N	0005515	molecular_function	protein binding
N	0005765	cellular_component	lysosomal membrane
N	0005829	cellular_component	cytosol
N	0005886	cellular_component	plasma membrane
N	0007035	biological_process	vacuolar acidification
N	0007042	biological_process	lysosomal lumen acidification
N	0010008	cellular_component	endosome membrane
N	0015078	molecular_function	proton transmembrane transporter activity
N	0016020	cellular_component	membrane
N	0016471	cellular_component	vacuolar proton-transporting V-type ATPase complex
N	0030665	cellular_component	clathrin-coated vesicle membrane
N	0030672	cellular_component	synaptic vesicle membrane
N	0031410	cellular_component	cytoplasmic vesicle
N	0033176	cellular_component	proton-transporting V-type ATPase complex
N	0033180	cellular_component	proton-transporting V-type ATPase, V1 domain
N	0034220	biological_process	monoatomic ion transmembrane transport
N	0042625	molecular_function	ATPase-coupled ion transmembrane transporter activity
N	0045202	cellular_component	synapse
N	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
N	0048388	biological_process	endosomal lumen acidification
N	0051452	biological_process	intracellular pH reduction
N	0061795	biological_process	Golgi lumen acidification
N	0070062	cellular_component	extracellular exosome
N	0097401	biological_process	synaptic vesicle lumen acidification
N	1902495	cellular_component	transmembrane transporter complex
N	1902600	biological_process	proton transmembrane transport
X	0005515	molecular_function	protein binding
Y	0005515	molecular_function	protein binding
Z	0005515	molecular_function	protein binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	binding site for residue ADP C 701

Chain	Residue
C	ALA251
C	ASN523
C	GLY524
C	TYR525
F	ARG400
F	LYS403
C	GLY253
C	CYS254
C	GLY255
C	LYS256
C	THR257
C	VAL258
C	GLU283
C	PHE445

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Functional Information from PROSITE/UniProt

site_id	PS00152
Number of Residues	10
Details	ATPASE_ALPHA_BETA ATP synthase alpha and beta subunits signature. PSVNWLISYS

Chain	Residue	Details
A	PRO446-SER455
D	PRO390-SER399

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	MOD_RES: N-acetylalanine => ECO:0000269|Ref.6

Chain	Residue	Details
M	ALA2
L	ALA2
K	ALA2

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site_id	SWS_FT_FI2
Number of Residues	3
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P50518

Chain	Residue	Details
J	TYR56
I	TYR56
H	TYR56

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	MOD_RES: Phosphoserine; by AMPK => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER384
B	SER384
C	SER384

---