6PAW

Crystal structure of DAPK2 S308A Calcium/Calmodulin complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation
B	0004672	molecular_function	protein kinase activity
B	0005524	molecular_function	ATP binding
B	0006468	biological_process	protein phosphorylation
C	0000086	biological_process	G2/M transition of mitotic cell cycle
C	0000922	cellular_component	spindle pole
C	0002027	biological_process	regulation of heart rate
C	0005509	molecular_function	calcium ion binding
C	0005513	biological_process	detection of calcium ion
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005813	cellular_component	centrosome
C	0005819	cellular_component	spindle
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005876	cellular_component	spindle microtubule
C	0005886	cellular_component	plasma membrane
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0008076	cellular_component	voltage-gated potassium channel complex
C	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
C	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
C	0010856	molecular_function	adenylate cyclase activator activity
C	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
C	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
C	0016020	cellular_component	membrane
C	0016240	biological_process	autophagosome membrane docking
C	0019855	molecular_function	calcium channel inhibitor activity
C	0019901	molecular_function	protein kinase binding
C	0021762	biological_process	substantia nigra development
C	0030017	cellular_component	sarcomere
C	0031432	molecular_function	titin binding
C	0031514	cellular_component	motile cilium
C	0031954	biological_process	positive regulation of protein autophosphorylation
C	0031982	cellular_component	vesicle
C	0032465	biological_process	regulation of cytokinesis
C	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
C	0032991	cellular_component	protein-containing complex
C	0034704	cellular_component	calcium channel complex
C	0035307	biological_process	positive regulation of protein dephosphorylation
C	0035458	biological_process	cellular response to interferon-beta
C	0043209	cellular_component	myelin sheath
C	0043539	molecular_function	protein serine/threonine kinase activator activity
C	0044325	molecular_function	transmembrane transporter binding
C	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
C	0046872	molecular_function	metal ion binding
C	0048306	molecular_function	calcium-dependent protein binding
C	0050848	biological_process	regulation of calcium-mediated signaling
C	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
C	0051592	biological_process	response to calcium ion
C	0055117	biological_process	regulation of cardiac muscle contraction
C	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
C	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
C	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
C	0071346	biological_process	cellular response to type II interferon
C	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
C	0072542	molecular_function	protein phosphatase activator activity
C	0097225	cellular_component	sperm midpiece
C	0098901	biological_process	regulation of cardiac muscle cell action potential
C	0140056	biological_process	organelle localization by membrane tethering
C	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
C	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
C	1902494	cellular_component	catalytic complex
C	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
C	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
D	0000086	biological_process	G2/M transition of mitotic cell cycle
D	0000922	cellular_component	spindle pole
D	0002027	biological_process	regulation of heart rate
D	0005509	molecular_function	calcium ion binding
D	0005513	biological_process	detection of calcium ion
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005813	cellular_component	centrosome
D	0005819	cellular_component	spindle
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0005876	cellular_component	spindle microtubule
D	0005886	cellular_component	plasma membrane
D	0007186	biological_process	G protein-coupled receptor signaling pathway
D	0008076	cellular_component	voltage-gated potassium channel complex
D	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
D	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
D	0010856	molecular_function	adenylate cyclase activator activity
D	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
D	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
D	0016020	cellular_component	membrane
D	0016240	biological_process	autophagosome membrane docking
D	0019855	molecular_function	calcium channel inhibitor activity
D	0019901	molecular_function	protein kinase binding
D	0021762	biological_process	substantia nigra development
D	0030017	cellular_component	sarcomere
D	0031432	molecular_function	titin binding
D	0031514	cellular_component	motile cilium
D	0031954	biological_process	positive regulation of protein autophosphorylation
D	0031982	cellular_component	vesicle
D	0032465	biological_process	regulation of cytokinesis
D	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
D	0032991	cellular_component	protein-containing complex
D	0034704	cellular_component	calcium channel complex
D	0035307	biological_process	positive regulation of protein dephosphorylation
D	0035458	biological_process	cellular response to interferon-beta
D	0043209	cellular_component	myelin sheath
D	0043539	molecular_function	protein serine/threonine kinase activator activity
D	0044325	molecular_function	transmembrane transporter binding
D	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
D	0046872	molecular_function	metal ion binding
D	0048306	molecular_function	calcium-dependent protein binding
D	0050848	biological_process	regulation of calcium-mediated signaling
D	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
D	0051592	biological_process	response to calcium ion
D	0055117	biological_process	regulation of cardiac muscle contraction
D	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
D	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
D	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
D	0071346	biological_process	cellular response to type II interferon
D	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
D	0072542	molecular_function	protein phosphatase activator activity
D	0097225	cellular_component	sperm midpiece
D	0098901	biological_process	regulation of cardiac muscle cell action potential
D	0140056	biological_process	organelle localization by membrane tethering
D	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
D	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
D	1902494	cellular_component	catalytic complex
D	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
D	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
E	0004672	molecular_function	protein kinase activity
E	0005524	molecular_function	ATP binding
E	0006468	biological_process	protein phosphorylation
F	0004672	molecular_function	protein kinase activity
F	0005524	molecular_function	ATP binding
F	0006468	biological_process	protein phosphorylation
G	0000086	biological_process	G2/M transition of mitotic cell cycle
G	0000922	cellular_component	spindle pole
G	0002027	biological_process	regulation of heart rate
G	0005509	molecular_function	calcium ion binding
G	0005513	biological_process	detection of calcium ion
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005813	cellular_component	centrosome
G	0005819	cellular_component	spindle
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0005876	cellular_component	spindle microtubule
G	0005886	cellular_component	plasma membrane
G	0007186	biological_process	G protein-coupled receptor signaling pathway
G	0008076	cellular_component	voltage-gated potassium channel complex
G	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
G	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
G	0010856	molecular_function	adenylate cyclase activator activity
G	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
G	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
G	0016020	cellular_component	membrane
G	0016240	biological_process	autophagosome membrane docking
G	0019855	molecular_function	calcium channel inhibitor activity
G	0019901	molecular_function	protein kinase binding
G	0021762	biological_process	substantia nigra development
G	0030017	cellular_component	sarcomere
G	0031432	molecular_function	titin binding
G	0031514	cellular_component	motile cilium
G	0031954	biological_process	positive regulation of protein autophosphorylation
G	0031982	cellular_component	vesicle
G	0032465	biological_process	regulation of cytokinesis
G	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
G	0032991	cellular_component	protein-containing complex
G	0034704	cellular_component	calcium channel complex
G	0035307	biological_process	positive regulation of protein dephosphorylation
G	0035458	biological_process	cellular response to interferon-beta
G	0043209	cellular_component	myelin sheath
G	0043539	molecular_function	protein serine/threonine kinase activator activity
G	0044325	molecular_function	transmembrane transporter binding
G	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
G	0046872	molecular_function	metal ion binding
G	0048306	molecular_function	calcium-dependent protein binding
G	0050848	biological_process	regulation of calcium-mediated signaling
G	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
G	0051592	biological_process	response to calcium ion
G	0055117	biological_process	regulation of cardiac muscle contraction
G	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
G	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
G	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
G	0071346	biological_process	cellular response to type II interferon
G	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
G	0072542	molecular_function	protein phosphatase activator activity
G	0097225	cellular_component	sperm midpiece
G	0098901	biological_process	regulation of cardiac muscle cell action potential
G	0140056	biological_process	organelle localization by membrane tethering
G	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
G	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
G	1902494	cellular_component	catalytic complex
G	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
G	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
H	0000086	biological_process	G2/M transition of mitotic cell cycle
H	0000922	cellular_component	spindle pole
H	0002027	biological_process	regulation of heart rate
H	0005509	molecular_function	calcium ion binding
H	0005513	biological_process	detection of calcium ion
H	0005515	molecular_function	protein binding
H	0005576	cellular_component	extracellular region
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005813	cellular_component	centrosome
H	0005819	cellular_component	spindle
H	0005829	cellular_component	cytosol
H	0005856	cellular_component	cytoskeleton
H	0005876	cellular_component	spindle microtubule
H	0005886	cellular_component	plasma membrane
H	0007186	biological_process	G protein-coupled receptor signaling pathway
H	0008076	cellular_component	voltage-gated potassium channel complex
H	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
H	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
H	0010856	molecular_function	adenylate cyclase activator activity
H	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
H	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
H	0016020	cellular_component	membrane
H	0016240	biological_process	autophagosome membrane docking
H	0019855	molecular_function	calcium channel inhibitor activity
H	0019901	molecular_function	protein kinase binding
H	0021762	biological_process	substantia nigra development
H	0030017	cellular_component	sarcomere
H	0031432	molecular_function	titin binding
H	0031514	cellular_component	motile cilium
H	0031954	biological_process	positive regulation of protein autophosphorylation
H	0031982	cellular_component	vesicle
H	0032465	biological_process	regulation of cytokinesis
H	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
H	0032991	cellular_component	protein-containing complex
H	0034704	cellular_component	calcium channel complex
H	0035307	biological_process	positive regulation of protein dephosphorylation
H	0035458	biological_process	cellular response to interferon-beta
H	0043209	cellular_component	myelin sheath
H	0043539	molecular_function	protein serine/threonine kinase activator activity
H	0044325	molecular_function	transmembrane transporter binding
H	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
H	0046872	molecular_function	metal ion binding
H	0048306	molecular_function	calcium-dependent protein binding
H	0050848	biological_process	regulation of calcium-mediated signaling
H	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
H	0051592	biological_process	response to calcium ion
H	0055117	biological_process	regulation of cardiac muscle contraction
H	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
H	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
H	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
H	0071346	biological_process	cellular response to type II interferon
H	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
H	0072542	molecular_function	protein phosphatase activator activity
H	0097225	cellular_component	sperm midpiece
H	0098901	biological_process	regulation of cardiac muscle cell action potential
H	0140056	biological_process	organelle localization by membrane tethering
H	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
H	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
H	1902494	cellular_component	catalytic complex
H	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
H	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue CA D 201

Chain	Residue
D	ASP21
D	ASP23
D	ASP25
D	THR27
D	GLU32

---

site_id	AC2
Number of Residues	6
Details	binding site for residue CA D 202

Chain	Residue
D	ASP65
D	GLU68
D	ASP57
D	ASP59
D	ASN61
D	THR63

---

site_id	AC3
Number of Residues	5
Details	binding site for residue CA D 203

Chain	Residue
D	ASP94
D	ASP96
D	ASN98
D	TYR100
D	GLU105

---

site_id	AC4
Number of Residues	6
Details	binding site for residue CA D 204

Chain	Residue
D	ASP130
D	ASP132
D	ASP134
D	GLN136
D	ASN138
D	GLU141

---

site_id	AC5
Number of Residues	5
Details	binding site for residue CA G 201

Chain	Residue
G	ASP96
G	ASN98
G	TYR100
G	GLU105
G	GLN136

---

site_id	AC6
Number of Residues	5
Details	binding site for residue CA G 202

Chain	Residue
G	ASP130
G	ASP132
G	ASP134
G	GLN136
G	GLU141

---

site_id	AC7
Number of Residues	6
Details	binding site for residue CA H 201

Chain	Residue
H	ASP130
H	ASP132
H	ASP134
H	GLN136
H	ASN138
H	GLU141

---

site_id	AC8
Number of Residues	5
Details	binding site for residue CA C 201

Chain	Residue
C	ASP94
C	ASP96
C	ASN98
C	TYR100
C	GLU105

---

site_id	AC9
Number of Residues	5
Details	binding site for residue CA C 202

Chain	Residue
C	ASP130
C	ASP132
C	ASP134
C	GLN136
C	GLU141

---

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL

Chain	Residue	Details
D	ASP21-LEU33
D	ASP57-PHE69
D	ASP94-LEU106
D	ASP130-PHE142

---

site_id	PS00107
Number of Residues	28
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGSGQFAIVKkCrekstgleyaak......FIKK

Chain	Residue	Details
A	LEU19-LYS46

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IaHfDLKpeNIML

Chain	Residue	Details
A	ILE135-LEU147

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	40
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:25441029, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:4UMO, ECO:0007744|PDB:4V0C, ECO:0007744|PDB:5J03

Chain	Residue	Details
D	ASP25
D	THR27
D	ASP21
D	GLU32
D	ASP23
H	ASP21
H	ASP23
H	ASP25
H	THR27
H	GLU32
H	ASP57
H	ASP59
H	ASN61
H	THR63
H	GLU68
C	ASP21
C	ASP23
C	ASP25
C	THR27
C	GLU32
C	ASP57
C	ASP59
C	ASN61
C	THR63
C	GLU68
D	ASP57
D	ASP59
D	ASN61
D	THR63
D	GLU68
G	ASP21
G	ASP23
G	ASP25
G	THR27
G	GLU32
G	ASP57
G	ASP59
G	ASN61
G	THR63
G	GLU68

---

site_id	SWS_FT_FI2
Number of Residues	40
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:5J03

Chain	Residue	Details
D	ASN98
H	ASP94
H	ASP96
H	ASN98
H	TYR100
H	GLU105
H	ASP130
H	ASP132
H	ASP134
H	GLN136
H	GLU141
C	ASP94
C	ASP96
C	ASN98
C	TYR100
C	GLU105
C	ASP130
C	ASP132
C	ASP134
C	GLN136
C	GLU141
D	ASP94
D	ASP96
D	TYR100
D	GLU105
D	ASP130
D	ASP132
D	ASP134
D	GLN136
D	GLU141
G	ASP94
G	ASP96
G	ASN98
G	TYR100
G	GLU105
G	ASP130
G	ASP132
G	ASP134
G	GLN136
G	GLU141

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: N-acetylalanine => ECO:0000269|PubMed:7093203, ECO:0000269|Ref.7, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712

Chain	Residue	Details
H	ALA2
D	ALA2
G	ALA2
C	ALA2

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861

Chain	Residue	Details
H	LYS22
D	LYS22
G	LYS22
C	LYS22

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphothreonine; by CaMK4 => ECO:0000250|UniProtKB:P0DP29

Chain	Residue	Details
H	THR45
C	THR45
D	THR45
G	THR45

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
G	SER82
D	SER82
H	SER82
C	SER82

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
G	LYS95
D	LYS95
H	LYS95
C	LYS95

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332

Chain	Residue	Details
D	TYR100
G	TYR100
H	TYR100
C	TYR100

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
G	SER102
D	SER102
H	SER102
C	SER102

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
D	THR111
G	THR111
H	THR111
C	THR111

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	MOD_RES: N6-methyllysine; alternate => ECO:0007744|PubMed:24129315

Chain	Residue	Details
D	LYS116
G	LYS116
H	LYS116
C	LYS116

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332

Chain	Residue	Details
G	TYR139
D	TYR139
H	TYR139
C	TYR139

---

site_id	SWS_FT_FI13
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157

Chain	Residue	Details
G	LYS22
H	LYS22
C	LYS22
D	LYS22

---