5UAT

Structure of human PYCR-1 complexed with NADPH

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004735	molecular_function	pyrroline-5-carboxylate reductase activity
A	0005515	molecular_function	protein binding
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0006561	biological_process	proline biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0034599	biological_process	cellular response to oxidative stress
A	0042802	molecular_function	identical protein binding
A	0051881	biological_process	regulation of mitochondrial membrane potential
A	0055129	biological_process	L-proline biosynthetic process
A	1903377	biological_process	negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
B	0004735	molecular_function	pyrroline-5-carboxylate reductase activity
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0006561	biological_process	proline biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0034599	biological_process	cellular response to oxidative stress
B	0042802	molecular_function	identical protein binding
B	0051881	biological_process	regulation of mitochondrial membrane potential
B	0055129	biological_process	L-proline biosynthetic process
B	1903377	biological_process	negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
C	0004735	molecular_function	pyrroline-5-carboxylate reductase activity
C	0005515	molecular_function	protein binding
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0006561	biological_process	proline biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0034599	biological_process	cellular response to oxidative stress
C	0042802	molecular_function	identical protein binding
C	0051881	biological_process	regulation of mitochondrial membrane potential
C	0055129	biological_process	L-proline biosynthetic process
C	1903377	biological_process	negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
D	0004735	molecular_function	pyrroline-5-carboxylate reductase activity
D	0005515	molecular_function	protein binding
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0006561	biological_process	proline biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
D	0034599	biological_process	cellular response to oxidative stress
D	0042802	molecular_function	identical protein binding
D	0051881	biological_process	regulation of mitochondrial membrane potential
D	0055129	biological_process	L-proline biosynthetic process
D	1903377	biological_process	negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
E	0004735	molecular_function	pyrroline-5-carboxylate reductase activity
E	0005515	molecular_function	protein binding
E	0005739	cellular_component	mitochondrion
E	0005759	cellular_component	mitochondrial matrix
E	0006561	biological_process	proline biosynthetic process
E	0016491	molecular_function	oxidoreductase activity
E	0034599	biological_process	cellular response to oxidative stress
E	0042802	molecular_function	identical protein binding
E	0051881	biological_process	regulation of mitochondrial membrane potential
E	0055129	biological_process	L-proline biosynthetic process
E	1903377	biological_process	negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	binding site for residue SO4 A 401

Chain	Residue
A	VAL231
A	SER233
A	GLY236
A	ALA237
A	THR238
A	HOH503
A	HOH513
A	HOH518
A	HOH524

---

site_id	AC2
Number of Residues	12
Details	binding site for residue SO4 B 401

Chain	Residue
A	ALA97
B	VAL231
B	SER233
B	GLY236
B	ALA237
B	THR238
B	HOH504
B	HOH512
B	HOH536
B	HOH547
B	HOH552
B	HOH575

---

site_id	AC3
Number of Residues	33
Details	binding site for residue NDP C 401

Chain	Residue
C	GLY7
C	ALA8
C	GLY9
C	GLN10
C	LEU11
C	SER34
C	PRO35
C	ASP36
C	MET37
C	ASN56
C	ALA69
C	VAL70
C	LYS71
C	PRO72
C	ILE78
C	CYS95
C	ALA96
C	ALA97
C	MET121
C	THR122
C	THR124
C	HOH515
C	HOH527
C	HOH536
C	HOH543
C	HOH553
C	HOH556
C	HOH565
C	HOH574
C	HOH589
D	ASN230
D	VAL231
D	SO4401

---

site_id	AC4
Number of Residues	8
Details	binding site for residue SO4 C 402

Chain	Residue
C	VAL231
C	SER233
C	GLY236
C	ALA237
C	THR238
C	HOH503
C	HOH546
C	HOH579

---

site_id	AC5
Number of Residues	2
Details	binding site for residue PEG C 403

Chain	Residue
B	ASP64
B	ARG89

---

site_id	AC6
Number of Residues	7
Details	binding site for residue SO4 D 401

Chain	Residue
C	NDP401
D	VAL231
D	SER233
D	ALA237
D	THR238
D	HOH532
D	HOH556

---

site_id	AC7
Number of Residues	29
Details	binding site for residue NDP E 401

Chain	Residue
E	HOH572
E	ALA8
E	GLY9
E	GLN10
E	LEU11
E	SER34
E	PRO35
E	ASP36
E	MET37
E	ASN56
E	ALA69
E	VAL70
E	LYS71
E	PRO72
E	ILE78
E	CYS95
E	ALA96
E	ALA97
E	MET121
E	THR122
E	THR124
E	ASN230
E	VAL231
E	HOH503
E	HOH508
E	HOH519
E	HOH551
E	HOH556
E	HOH559

---

Functional Information from PROSITE/UniProt

site_id	PS00521
Number of Residues	23
Details	P5CR Delta 1-pyrroline-5-carboxylate reductase signature. Pgq.LkdnVSSpGGaTihALhvLE

Chain	Residue	Details
A	PRO224-GLU246

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	25
Details	BINDING: BINDING => ECO:0000269|Ref.18

Chain	Residue	Details
A	ILE6
B	CYS95
C	ILE6
C	SER34
C	ASN56
C	ALA69
C	CYS95
D	ILE6
D	SER34
D	ASN56
D	ALA69
A	SER34
D	CYS95
E	ILE6
E	SER34
E	ASN56
E	ALA69
E	CYS95
A	ASN56
A	ALA69
A	CYS95
B	ILE6
B	SER34
B	ASN56
B	ALA69

---

site_id	SWS_FT_FI2
Number of Residues	5
Details	MOD_RES: N-acetylserine => ECO:0000269|Ref.8, ECO:0000269|Ref.9, ECO:0007744|PubMed:19413330

Chain	Residue	Details
A	SER2
B	SER2
C	SER2
D	SER2
E	SER2

---

site_id	SWS_FT_FI3
Number of Residues	5
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER278
B	SER278
C	SER278
D	SER278
E	SER278

---