5JNW

Crystal structure of bovine low molecular weight protein tyrosine phosphatase (LMPTP) mutant (W49Y N50E) complexed with vanadate and uncompetitive inhibitor

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003993	molecular_function	acid phosphatase activity
A	0004721	molecular_function	phosphoprotein phosphatase activity
A	0004725	molecular_function	protein tyrosine phosphatase activity
A	0004726	molecular_function	non-membrane spanning protein tyrosine phosphatase activity
A	0005737	cellular_component	cytoplasm
A	0006470	biological_process	protein dephosphorylation
A	0016791	molecular_function	phosphatase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue VO4 A 201

Chain	Residue
A	CYS12
A	LEU13
A	GLY14
A	ASN15
A	ILE16
A	CYS17
A	ARG18
A	6LJ202

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site_id	AC2
Number of Residues	8
Details	binding site for residue 6LJ A 202

Chain	Residue
A	TYR49
A	GLU50
A	ARG53
A	ASP129
A	TYR131
A	TYR132
A	VO4201
A	ILE16

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:8052313, ECO:0000269|PubMed:8132604, ECO:0000269|PubMed:8319676

Chain	Residue	Details
A	CYS12

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:8052313, ECO:0000269|PubMed:8319676

Chain	Residue	Details
A	ARG18

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site_id	SWS_FT_FI3
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000269|PubMed:8052313

Chain	Residue	Details
A	ASP129

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0000269|PubMed:2644264

Chain	Residue	Details
A	ALA1

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site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P24666

Chain	Residue	Details
A	TYR131
A	TYR132

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 462

Chain	Residue	Details
A	CYS12	covalent catalysis
A	ASN15	activator, electrostatic stabiliser
A	CYS17	steric role
A	ARG18	transition state stabiliser
A	SER19	activator, electrostatic stabiliser
A	ASP129	proton shuttle (general acid/base)

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