Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4OV2

Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus norvegicus

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0005245	molecular_function	voltage-gated calcium channel activity
A	0005509	molecular_function	calcium ion binding
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005794	cellular_component	Golgi apparatus
A	0005886	cellular_component	plasma membrane
A	0008048	molecular_function	calcium sensitive guanylate cyclase activator activity
A	0008427	molecular_function	calcium-dependent protein kinase inhibitor activity
A	0010975	biological_process	regulation of neuron projection development
A	0014069	cellular_component	postsynaptic density
A	0019901	molecular_function	protein kinase binding
A	0030424	cellular_component	axon
A	0030425	cellular_component	dendrite
A	0031045	cellular_component	dense core granule
A	0044305	cellular_component	calyx of Held
A	0045202	cellular_component	synapse
A	0045921	biological_process	positive regulation of exocytosis
A	0046872	molecular_function	metal ion binding
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0050850	biological_process	positive regulation of calcium-mediated signaling
A	0070588	biological_process	calcium ion transmembrane transport
A	0098794	cellular_component	postsynapse
A	0098978	cellular_component	glutamatergic synapse
A	0099509	biological_process	regulation of presynaptic cytosolic calcium ion concentration
A	0099523	cellular_component	presynaptic cytosol
A	0099524	cellular_component	postsynaptic cytosol
A	2000300	biological_process	regulation of synaptic vesicle exocytosis
B	0000287	molecular_function	magnesium ion binding
B	0005245	molecular_function	voltage-gated calcium channel activity
B	0005509	molecular_function	calcium ion binding
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005794	cellular_component	Golgi apparatus
B	0005886	cellular_component	plasma membrane
B	0008048	molecular_function	calcium sensitive guanylate cyclase activator activity
B	0008427	molecular_function	calcium-dependent protein kinase inhibitor activity
B	0010975	biological_process	regulation of neuron projection development
B	0014069	cellular_component	postsynaptic density
B	0019901	molecular_function	protein kinase binding
B	0030424	cellular_component	axon
B	0030425	cellular_component	dendrite
B	0031045	cellular_component	dense core granule
B	0044305	cellular_component	calyx of Held
B	0045202	cellular_component	synapse
B	0045921	biological_process	positive regulation of exocytosis
B	0046872	molecular_function	metal ion binding
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0050850	biological_process	positive regulation of calcium-mediated signaling
B	0070588	biological_process	calcium ion transmembrane transport
B	0098794	cellular_component	postsynapse
B	0098978	cellular_component	glutamatergic synapse
B	0099509	biological_process	regulation of presynaptic cytosolic calcium ion concentration
B	0099523	cellular_component	presynaptic cytosol
B	0099524	cellular_component	postsynaptic cytosol
B	2000300	biological_process	regulation of synaptic vesicle exocytosis
C	0000287	molecular_function	magnesium ion binding
C	0005245	molecular_function	voltage-gated calcium channel activity
C	0005509	molecular_function	calcium ion binding
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005794	cellular_component	Golgi apparatus
C	0005886	cellular_component	plasma membrane
C	0008048	molecular_function	calcium sensitive guanylate cyclase activator activity
C	0008427	molecular_function	calcium-dependent protein kinase inhibitor activity
C	0010975	biological_process	regulation of neuron projection development
C	0014069	cellular_component	postsynaptic density
C	0019901	molecular_function	protein kinase binding
C	0030424	cellular_component	axon
C	0030425	cellular_component	dendrite
C	0031045	cellular_component	dense core granule
C	0044305	cellular_component	calyx of Held
C	0045202	cellular_component	synapse
C	0045921	biological_process	positive regulation of exocytosis
C	0046872	molecular_function	metal ion binding
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0050850	biological_process	positive regulation of calcium-mediated signaling
C	0070588	biological_process	calcium ion transmembrane transport
C	0098794	cellular_component	postsynapse
C	0098978	cellular_component	glutamatergic synapse
C	0099509	biological_process	regulation of presynaptic cytosolic calcium ion concentration
C	0099523	cellular_component	presynaptic cytosol
C	0099524	cellular_component	postsynaptic cytosol
C	2000300	biological_process	regulation of synaptic vesicle exocytosis
D	0000287	molecular_function	magnesium ion binding
D	0005245	molecular_function	voltage-gated calcium channel activity
D	0005509	molecular_function	calcium ion binding
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005794	cellular_component	Golgi apparatus
D	0005886	cellular_component	plasma membrane
D	0008048	molecular_function	calcium sensitive guanylate cyclase activator activity
D	0008427	molecular_function	calcium-dependent protein kinase inhibitor activity
D	0010975	biological_process	regulation of neuron projection development
D	0014069	cellular_component	postsynaptic density
D	0019901	molecular_function	protein kinase binding
D	0030424	cellular_component	axon
D	0030425	cellular_component	dendrite
D	0031045	cellular_component	dense core granule
D	0044305	cellular_component	calyx of Held
D	0045202	cellular_component	synapse
D	0045921	biological_process	positive regulation of exocytosis
D	0046872	molecular_function	metal ion binding
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0050850	biological_process	positive regulation of calcium-mediated signaling
D	0070588	biological_process	calcium ion transmembrane transport
D	0098794	cellular_component	postsynapse
D	0098978	cellular_component	glutamatergic synapse
D	0099509	biological_process	regulation of presynaptic cytosolic calcium ion concentration
D	0099523	cellular_component	presynaptic cytosol
D	0099524	cellular_component	postsynaptic cytosol
D	2000300	biological_process	regulation of synaptic vesicle exocytosis

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 201

Chain	Residue
A	ASP73
A	ASN75
A	ASP77
A	ARG79
A	GLU84

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 202

Chain	Residue
A	GLU120
A	ASP109
A	ASP111
A	ASP113
A	TYR115

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 203

Chain	Residue
A	ASP157
A	ASN159
A	ASP161
A	LYS163
A	GLU168

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA B 201

Chain	Residue
B	ASP73
B	ASN75
B	ASP77
B	ARG79
B	GLU84

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA B 202

Chain	Residue
B	ASP109
B	ASP111
B	ASP113
B	TYR115
B	GLU120
B	HOH301

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA B 203

Chain	Residue
B	ASP157
B	ASN159
B	ASP161
B	LYS163
B	GLU168
D	HOH308

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA C 201

Chain	Residue
C	ASP73
C	ASN75
C	ASP77
C	ARG79
C	GLU81
C	GLU84

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA C 202

Chain	Residue
C	ASP109
C	ASP111
C	ASP113
C	TYR115
C	GLU120

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA C 203

Chain	Residue
C	ASP157
C	ASN159
C	ASP161
C	LYS163
C	GLU168

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA D 201

Chain	Residue
D	ASP73
D	ASN75
D	ASP77
D	ARG79
D	GLU81
D	GLU84

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA D 202

Chain	Residue
D	ASP109
D	ASP111
D	ASP113
D	TYR115
D	GLU120

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA D 203

Chain	Residue
D	ASP157
D	ASN159
D	ASP161
D	LYS163
D	GLU168

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DENKDGRIEfsEF

Chain	Residue	Details
A	ASP73-PHE85
A	ASP109-MET121
A	ASP157-PHE169

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	20
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU00448, ECO:0000269\|PubMed:25979333, ECO:0007744\|PDB:4OV2, ECO:0007744\|PDB:4YRU, ECO:0007744\|PDB:5AEQ, ECO:0007744\|PDB:5AER

Chain	Residue	Details
A	ASP73
A	ASN75
A	ASP77
D	ASN75
D	ASP77
D	ARG79
D	GLU84
A	ARG79
A	GLU84
B	ASP73
B	ASN75
B	ASP77
B	ARG79
B	GLU84
C	ASP73
C	ASN75
C	ASP77
C	ARG79
C	GLU84
D	ASP73

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:25979333, ECO:0007744\|PDB:4YRU, ECO:0007744\|PDB:5AEQ, ECO:0007744\|PDB:5AER

Chain	Residue	Details
A	GLU81
B	GLU81
C	GLU81
D	GLU81

site_id	SWS_FT_FI3
Number of Residues	20
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU00448, ECO:0000269\|PubMed:25979333, ECO:0007744\|PDB:4OV2, ECO:0007744\|PDB:4YRU, ECO:0007744\|PDB:5AEQ

Chain	Residue	Details
A	GLU120
B	ASP109
B	ASP111
B	ASP113
B	TYR115
B	GLU120
C	ASP109
C	ASP111
C	ASP113
C	TYR115
C	GLU120
D	ASP109
D	ASP111
D	ASP113
D	TYR115
D	GLU120
A	ASP109
A	ASP111
A	ASP113
A	TYR115

site_id	SWS_FT_FI4
Number of Residues	20
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU00448, ECO:0000269\|PubMed:25979333, ECO:0007744\|PDB:4OV2, ECO:0007744\|PDB:4YRU, ECO:0007744\|PDB:5AEQ, ECO:0007744\|PDB:5AER, ECO:0007744\|PDB:5AFP

Chain	Residue	Details
D	ASN159
D	ASP161
D	LYS163
D	GLU168
A	ASN159
A	ASP161
A	LYS163
A	GLU168
B	LYS163
B	GLU168
C	ASP157
C	ASN159
C	ASP161
C	LYS163
C	GLU168
D	ASP157
A	ASP157
B	ASP157
B	ASN159
B	ASP161

site_id	SWS_FT_FI5
Number of Residues	4
Details	LIPID: N-myristoyl glycine => ECO:0000269\|PubMed:10514519

Chain	Residue	Details
A	GLY2
C	GLY2
D	GLY2
B	GLY2

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)