Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4I5L

Structural mechanism of trimeric PP2A holoenzyme involving PR70: insight for Cdc6 dephosphorylation

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000159	cellular_component	protein phosphatase type 2A complex
A	0000775	cellular_component	chromosome, centromeric region
A	0004722	molecular_function	protein serine/threonine phosphatase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005694	cellular_component	chromosome
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0007059	biological_process	chromosome segregation
A	0007143	biological_process	female meiotic nuclear division
A	0015630	cellular_component	microtubule cytoskeleton
A	0016020	cellular_component	membrane
A	0016328	cellular_component	lateral plasma membrane
A	0019888	molecular_function	protein phosphatase regulator activity
A	0030425	cellular_component	dendrite
A	0035331	biological_process	negative regulation of hippo signaling
A	0035556	biological_process	intracellular signal transduction
A	0040008	biological_process	regulation of growth
A	0042995	cellular_component	cell projection
A	0043005	cellular_component	neuron projection
A	0043025	cellular_component	neuronal cell body
A	0043029	biological_process	T cell homeostasis
A	0045202	cellular_component	synapse
A	0045595	biological_process	regulation of cell differentiation
A	0046982	molecular_function	protein heterodimerization activity
A	0051232	biological_process	meiotic spindle elongation
A	0051306	biological_process	mitotic sister chromatid separation
A	0051754	biological_process	meiotic sister chromatid cohesion, centromeric
A	0051898	biological_process	negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
A	0065003	biological_process	protein-containing complex assembly
A	0070062	cellular_component	extracellular exosome
A	0090443	cellular_component	FAR/SIN/STRIPAK complex
A	0098978	cellular_component	glutamatergic synapse
A	1903538	biological_process	regulation of meiotic cell cycle process involved in oocyte maturation
A	1990405	molecular_function	protein antigen binding
A	2001241	biological_process	positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
B	0005509	molecular_function	calcium ion binding
C	0000159	cellular_component	protein phosphatase type 2A complex
C	0000278	biological_process	mitotic cell cycle
C	0000775	cellular_component	chromosome, centromeric region
C	0000922	cellular_component	spindle pole
C	0001932	biological_process	regulation of protein phosphorylation
C	0004721	molecular_function	phosphoprotein phosphatase activity
C	0004722	molecular_function	protein serine/threonine phosphatase activity
C	0004725	molecular_function	protein tyrosine phosphatase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005694	cellular_component	chromosome
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005886	cellular_component	plasma membrane
C	0006470	biological_process	protein dephosphorylation
C	0007498	biological_process	mesoderm development
C	0008287	cellular_component	protein serine/threonine phosphatase complex
C	0010288	biological_process	response to lead ion
C	0010719	biological_process	negative regulation of epithelial to mesenchymal transition
C	0015630	cellular_component	microtubule cytoskeleton
C	0016020	cellular_component	membrane
C	0016787	molecular_function	hydrolase activity
C	0017018	molecular_function	myosin phosphatase activity
C	0035331	biological_process	negative regulation of hippo signaling
C	0035556	biological_process	intracellular signal transduction
C	0035970	biological_process	peptidyl-threonine dephosphorylation
C	0040008	biological_process	regulation of growth
C	0043029	biological_process	T cell homeostasis
C	0045121	cellular_component	membrane raft
C	0045202	cellular_component	synapse
C	0045595	biological_process	regulation of cell differentiation
C	0046872	molecular_function	metal ion binding
C	0046982	molecular_function	protein heterodimerization activity
C	0048156	molecular_function	tau protein binding
C	0050811	molecular_function	GABA receptor binding
C	0051321	biological_process	meiotic cell cycle
C	0051898	biological_process	negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
C	0070062	cellular_component	extracellular exosome
C	0070262	biological_process	peptidyl-serine dephosphorylation
C	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
C	0090443	cellular_component	FAR/SIN/STRIPAK complex
C	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
C	1904526	biological_process	regulation of microtubule binding
C	1904528	biological_process	positive regulation of microtubule binding
C	1904539	biological_process	negative regulation of glycolytic process through fructose-6-phosphate
C	2000045	biological_process	regulation of G1/S transition of mitotic cell cycle
D	0000159	cellular_component	protein phosphatase type 2A complex
D	0000775	cellular_component	chromosome, centromeric region
D	0004722	molecular_function	protein serine/threonine phosphatase activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005694	cellular_component	chromosome
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0007059	biological_process	chromosome segregation
D	0007143	biological_process	female meiotic nuclear division
D	0015630	cellular_component	microtubule cytoskeleton
D	0016020	cellular_component	membrane
D	0016328	cellular_component	lateral plasma membrane
D	0019888	molecular_function	protein phosphatase regulator activity
D	0030425	cellular_component	dendrite
D	0035331	biological_process	negative regulation of hippo signaling
D	0035556	biological_process	intracellular signal transduction
D	0040008	biological_process	regulation of growth
D	0042995	cellular_component	cell projection
D	0043005	cellular_component	neuron projection
D	0043025	cellular_component	neuronal cell body
D	0043029	biological_process	T cell homeostasis
D	0045202	cellular_component	synapse
D	0045595	biological_process	regulation of cell differentiation
D	0046982	molecular_function	protein heterodimerization activity
D	0051232	biological_process	meiotic spindle elongation
D	0051306	biological_process	mitotic sister chromatid separation
D	0051754	biological_process	meiotic sister chromatid cohesion, centromeric
D	0051898	biological_process	negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
D	0065003	biological_process	protein-containing complex assembly
D	0070062	cellular_component	extracellular exosome
D	0090443	cellular_component	FAR/SIN/STRIPAK complex
D	0098978	cellular_component	glutamatergic synapse
D	1903538	biological_process	regulation of meiotic cell cycle process involved in oocyte maturation
D	1990405	molecular_function	protein antigen binding
D	2001241	biological_process	positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
E	0005509	molecular_function	calcium ion binding
F	0000159	cellular_component	protein phosphatase type 2A complex
F	0000278	biological_process	mitotic cell cycle
F	0000775	cellular_component	chromosome, centromeric region
F	0000922	cellular_component	spindle pole
F	0001932	biological_process	regulation of protein phosphorylation
F	0004721	molecular_function	phosphoprotein phosphatase activity
F	0004722	molecular_function	protein serine/threonine phosphatase activity
F	0004725	molecular_function	protein tyrosine phosphatase activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005694	cellular_component	chromosome
F	0005737	cellular_component	cytoplasm
F	0005739	cellular_component	mitochondrion
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005886	cellular_component	plasma membrane
F	0006470	biological_process	protein dephosphorylation
F	0007498	biological_process	mesoderm development
F	0008287	cellular_component	protein serine/threonine phosphatase complex
F	0010288	biological_process	response to lead ion
F	0010719	biological_process	negative regulation of epithelial to mesenchymal transition
F	0015630	cellular_component	microtubule cytoskeleton
F	0016020	cellular_component	membrane
F	0016787	molecular_function	hydrolase activity
F	0017018	molecular_function	myosin phosphatase activity
F	0035331	biological_process	negative regulation of hippo signaling
F	0035556	biological_process	intracellular signal transduction
F	0035970	biological_process	peptidyl-threonine dephosphorylation
F	0040008	biological_process	regulation of growth
F	0043029	biological_process	T cell homeostasis
F	0045121	cellular_component	membrane raft
F	0045202	cellular_component	synapse
F	0045595	biological_process	regulation of cell differentiation
F	0046872	molecular_function	metal ion binding
F	0046982	molecular_function	protein heterodimerization activity
F	0048156	molecular_function	tau protein binding
F	0050811	molecular_function	GABA receptor binding
F	0051321	biological_process	meiotic cell cycle
F	0051898	biological_process	negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
F	0070062	cellular_component	extracellular exosome
F	0070262	biological_process	peptidyl-serine dephosphorylation
F	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
F	0090443	cellular_component	FAR/SIN/STRIPAK complex
F	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
F	1904526	biological_process	regulation of microtubule binding
F	1904528	biological_process	positive regulation of microtubule binding
F	1904539	biological_process	negative regulation of glycolytic process through fructose-6-phosphate
F	2000045	biological_process	regulation of G1/S transition of mitotic cell cycle

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PEG A 601

Chain	Residue
A	GLU376
A	ARG381
A	GLU410
A	ASP414
A	LYS416

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PEG A 602

Chain	Residue
A	SER214
A	ASP254
A	LYS255

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PEG A 603

Chain	Residue
A	LYS519
A	HIS520
F	HIS230
A	HIS479

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PEG A 604

Chain	Residue
A	ALA136
A	ARG144
A	TYR169
A	ASN172
A	ASP176
A	HOH765
E	PRO224

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA B 601

Chain	Residue
B	ASP401
B	ASP403
B	ASP405
B	ALA407
B	GLU412
B	HOH742

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA B 602

Chain	Residue
B	ASP327
B	ASP329
B	ASP331
B	LEU333
B	HOH740
B	HOH762

site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PEG B 603

Chain	Residue
B	SER259
B	THR263

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PEG B 604

Chain	Residue
B	ARG362
B	LYS365
B	VAL366
B	ASP376

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN C 401

Chain	Residue
C	ASP85
C	ASN117
C	HIS167
C	HIS241
C	MN402
C	HOH559

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN C 402

Chain	Residue
C	ASP57
C	HIS59
C	ASP85
C	MN401
C	HOH559
H	HOH104

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PEG C 403

Chain	Residue
B	LEU444
B	LEU463
C	TYR91
C	ARG135
C	LYS136

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PEG C 404

Chain	Residue
C	THR40
C	SER43
C	ASN44
C	ARG181
C	ARG185

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MLI C 405

Chain	Residue
C	ASN18
C	PHE69
C	LYS74
C	SER75
C	LEU99
C	HOH557

site_id	BC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PEG D 601

Chain	Residue
D	GLU297
D	HOH714

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PEG D 602

Chain	Residue
D	TYR456
D	ASN539
D	LYS542
F	ASP77

site_id	BC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA E 701

Chain	Residue
E	ASP401
E	ASP403
E	ASP405
E	ALA407
E	GLU412
E	HOH838

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA E 702

Chain	Residue
E	ASP327
E	ASP329
E	ASP331
E	LEU333
E	HOH873

site_id	BC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PEG E 703

Chain	Residue
E	GLN231
E	GLU232
E	SER259
E	THR263

site_id	CC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MLI E 704

Chain	Residue
E	GLY180
E	GLU189
E	PRO236
E	GLN239
E	ASP240
E	ASN243

site_id	CC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN F 401

Chain	Residue
F	ASP85
F	ASN117
F	HIS167
F	HIS241
F	MN402
F	HOH547

site_id	CC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN F 402

Chain	Residue
F	HIS59
F	ASP85
F	MN401
F	HOH546
F	HOH547
F	ASP57

site_id	CC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MLI F 403

Chain	Residue
F	ASN18
F	PHE69
F	LYS74
F	HOH519

site_id	CC5
Number of Residues	13
Details	BINDING SITE FOR CHAIN G OF MICROCYSTIN-LR (MCLR) BOUND FORM

Chain	Residue
F	ARG89
F	TYR127
F	VAL189
F	PRO190
F	HIS191
F	TRP200
F	PRO213
F	ARG214
F	TYR265
F	CYS266
F	CYS269
F	HOH546
F	HOH548

site_id	CC6
Number of Residues	15
Details	BINDING SITE FOR CHAIN H OF MICROCYSTIN-LR (MCLR) BOUND FORM

Chain	Residue
C	ARG89
C	ILE123
C	TYR127
C	VAL189
C	PRO190
C	HIS191
C	TRP200
C	PRO213
C	ARG214
C	TYR265
C	CYS266
C	CYS269
C	HOH532
H	HOH101
H	HOH104

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DLDGDGALSmfEL

Chain	Residue	Details
B	ASP401-LEU413

site_id	PS00125
Number of Residues	6
Details	SER_THR_PHOSPHATASE Serine/threonine specific protein phosphatases signature. LRGNHE

Chain	Residue	Details
C	LEU114-GLU119

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton donor => ECO:0000250\|UniProtKB:P36873

Chain	Residue	Details
B	ASP405
B	GLU412
E	ASP401
E	ASP403
E	ASP405
E	GLU412
C	HIS118
F	HIS118

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:17055435

Chain	Residue	Details
C	HIS167
C	HIS241
F	ASP57
F	HIS59
F	ASP85
F	ASN117
F	HIS167
F	HIS241
C	ASP57
C	HIS59
C	ASP85
C	ASN117

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250\|UniProtKB:P67774

Chain	Residue	Details
C	TYR307
F	TYR307

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Leucine methyl ester => ECO:0000269\|PubMed:8206937

Chain	Residue	Details
C	LEU309
F	LEU309

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)