8XU4
The Crystal Structure of MAPK2 from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-10 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.953727 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 139.812, 179.015, 212.397 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.953 - 3.400 |
Rwork | 0.214 |
R-free | 0.26310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.348 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.953 | 3.470 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.148 | 1.030 |
Number of reflections | 73900 | 5174 |
<I/σ(I)> | 13 | |
Completeness [%] | 100.0 | |
Redundancy | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.1M Sodium malonate dibasic monohydrate, 0.1M HEPES pH7, 0.5% v/v Jeffamine ED2003 |