8UDU
The X-RAY co-crystal structure of human FGFR3 and Compound 17
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-27 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.700, 69.314, 78.473 |
Unit cell angles | 90.00, 90.96, 90.00 |
Refinement procedure
Resolution | 34.140 - 1.737 |
R-factor | 0.2311 |
Rwork | 0.230 |
R-free | 0.25580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.780 |
Data reduction software | autoPROC (1.1.7) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.140 | 1.800 |
High resolution limit [Å] | 1.737 | 1.737 |
Rmerge | 0.040 | 0.910 |
Rmeas | 0.040 | 0.950 |
Rpim | 0.010 | 0.260 |
Number of reflections | 61639 | 6122 |
<I/σ(I)> | 26.9 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14 | 13.5 |
CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.10 M Magnesium Chloride, 0.10 M TRIS/HCl pH 8.10, 0.20 M Sodium Chloride, 20.00 % (w/v) PEG 3350 |