8RRQ
Crystal structure of human SYK in complex with compound 24
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.204, 87.344, 40.048 |
| Unit cell angles | 90.00, 92.16, 90.00 |
Refinement procedure
| Resolution | 43.670 - 1.600 |
| Rwork | 0.177 |
| R-free | 0.21070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.748 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.670 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.019 | 0.318 |
| Number of reflections | 34990 | 1625 |
| <I/σ(I)> | 17.4 | 2.2 |
| Completeness [%] | 98.7 | 91.9 |
| Redundancy | 2 | 2 |
| CC(1/2) | 1.000 | 0.823 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | 20% PEG 3350, 0.1 M bis tris propane pH 6.5, 0.2 M Na formate |
