8OO5

Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	81.577, 81.577, 234.132
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.430 - 2.250
R-factor	0.1826
Rwork	0.181
R-free	0.22018
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	2.079
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.430	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.271	2.748
Rmeas	0.278	2.810
Rpim	0.059	0.583
Total number of observations		47398
Number of reflections	22902	2066
<I/σ(I)>	11.4	2.2
Completeness [%]	100.0
Redundancy	21.8	22.9
CC(1/2)	0.996	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	1.91M LiSO4, 0.1M Tris pH 7.5