8FN8
Crystal structure of the C-terminal Fg domain of TNC with the single FN domain of PTPRZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.707, 61.520, 92.428 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.880 - 1.890 |
R-factor | 0.1672 |
Rwork | 0.164 |
R-free | 0.20530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 2J3F & 1FNA |
RMSD bond length | 0.003 |
RMSD bond angle | 0.667 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.960 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.113 | 0.342 |
Rmeas | 0.126 | 0.384 |
Rpim | 0.054 | 0.172 |
Number of reflections | 25470 | 2506 |
<I/σ(I)> | 16.2 | 4.7 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 4.9 | 4.3 |
CC(1/2) | 0.996 | 0.949 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% w/v PEG3350, 100 mM Tris-HCl, pH 8.0 |