8F5T

Rabbit muscle pyruvate kinase in complex with sodium and magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	142.453, 113.759, 169.144
Unit cell angles	90.00, 94.05, 90.00

Refinement procedure

Resolution	47.160 - 2.410
R-factor	0.1872
Rwork	0.185
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g50
RMSD bond length	0.002
RMSD bond angle	0.503
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.090	0.041	0.478
Total number of observations	870606
Number of reflections	397612	40558	38556
<I/σ(I)>	10.9
Completeness [%]	97.4	99.4	94.3
Redundancy	2.2	2.3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	50 mM Tris 50 mM NaCl and a range of 7.5 to 10% PEG 20,000