8ENK
Crystal structure of UAP56 in complex with Tho1, the yeast homolog of human SARNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.534, 96.786, 126.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.720 - 2.500 |
R-factor | 0.2253 |
Rwork | 0.224 |
R-free | 0.25290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xtj |
RMSD bond length | 0.003 |
RMSD bond angle | 0.695 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.071 | 0.918 |
Rpim | 0.031 | 0.396 |
Number of reflections | 38274 | 1873 |
<I/σ(I)> | 28.7 | 1.9 |
Completeness [%] | 99.9 | |
Redundancy | 6.2 | |
CC(1/2) | 0.999 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.025 M HEPES, pH 7.4, 0.2 M sodium chloride, and 18% PEG4000 |