8DIT
Cryo-EM structure of a HOPS core complex containing Vps33, Vps16, and Vps18
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.03 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.1 |
| Number of Particle | 4092244 |
| Software | cryoSPARC,RELION,EPU |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
