8D4R
Crystal Structure of Mosaic HIV-1 Envelope (MosM3.2) in Complex with antibodies PGT124 and 35O22 at 3.8 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03316 |
Spacegroup name | P 63 |
Unit cell lengths | 125.474, 125.474, 315.834 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.190 - 3.810 |
R-factor | 0.2788 |
Rwork | 0.277 |
R-free | 0.29590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cez |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.870 |
High resolution limit [Å] | 3.800 | 10.290 | 3.800 |
Rmerge | 0.163 | 0.036 | 1.206 |
Rmeas | 0.188 | 0.042 | 1.425 |
Rpim | 0.093 | 0.020 | 0.747 |
Total number of observations | 105995 | ||
Number of reflections | 27342 | 1365 | 1327 |
<I/σ(I)> | 5.1 | ||
Completeness [%] | 99.3 | 96.6 | 98.7 |
Redundancy | 3.9 | 3.9 | 3.4 |
CC(1/2) | 0.998 | 0.524 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris (pH=7.4), 23% v/v PEG 400 |