8A5I
Cryo-EM structure of Lincomycin bound to the Listeria monocytogenes 50S ribosomal subunit.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.3 |
| Number of Particle | 506262 |
| Software | RELION,EPU,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
