7ZH9
Uba1 in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 107.720, 118.064, 196.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.238 - 1.720 |
| R-factor | 0.1669 |
| Rwork | 0.165 |
| R-free | 0.20610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cmm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.924 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.238 | 1.705 |
| High resolution limit [Å] | 1.705 | 1.705 |
| Number of reflections | 91872 | 4617 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.65 | |
| CC(1/2) | 0.999 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M ammonium sulfate, 0.1 M HEPES pH 7.5 and 25% PEG 3350 |
