7Z4V

Structure of Serine-Threonine kinase STK25 in complex with compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	126.200, 46.413, 57.954
Unit cell angles	90.00, 90.56, 90.00

Refinement procedure

Resolution	63.100 - 1.644
R-factor	0.247
Rwork	0.245
R-free	0.28340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2kix
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.100	1.828
High resolution limit [Å]	1.650	1.650
Rmerge	0.101	1.123
Rmeas	0.121
Rpim	0.065
Number of reflections	27829	1391
<I/σ(I)>	10
Completeness [%]	92.3	65.7
Redundancy	6.5
CC(1/2)	0.995	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	22 % PEG 3350, 0.1 M Bis-tris propane pH 8.5, 0.2 M Potassium citrate, 10 % ethylene glycol