7YXU
Crystal structure of agonistic antibody 1618 fab domain bound to human 4-1BB.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 65.064, 213.465, 54.012 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.010 | 2.370 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.110 | |
Number of reflections | 34026 | 2403 |
<I/σ(I)> | 8.9 | |
Completeness [%] | 99.9 | |
Redundancy | 6.5 | |
CC(1/2) | 0.970 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris/HCl pH 8.5, 0.2 M trimethyloxide (TMAO), 20% (w/v) PEG 2000 monomethylether (MME) and 20 mM MnCl2 |