7VYX
Crystal structure of the selenomethionine(SeMet)-derived Cas12c1 (D969A) ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-12-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.127, 149.850, 173.246 |
Unit cell angles | 90.00, 91.59, 90.00 |
Refinement procedure
Resolution | 49.730 - 3.200 |
R-factor | 0.2575 |
Rwork | 0.256 |
R-free | 0.29240 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.13-2998) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.130 | 1.634 |
Number of reflections | 78406 | 7804 |
<I/σ(I)> | 14.8 | 1.18 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.8 | 7 |
CC(1/2) | 0.996 | 0.493 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M Tris-HCl (pH 8.2), 16% polyethylene glycol 3350 (w/v), 0.02 M citric acid, 0.05 M lithium acetate dihydrate |