7UGM

Crystal Structure of BG24-iGL CDR3mat Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	53.254, 70.835, 134.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.000 - 1.400
Rwork	0.193
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7uce
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	38.000
High resolution limit [Å]	1.400	1.400
Number of reflections	101139	101139
<I/σ(I)>	11
Completeness [%]	100.0
Redundancy	19.7
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350